Bronchial Asthma
Conditions
Brief summary
Bronchial asthma affects more than a quarter of a billion people worldwide. It is responsible for over 1000 deaths a day, of which the majority are preventable (Levy, 2015). Asthma is a common heterogeneous disease characterized by chronic airway inflammation. It is defined by the history of variable respiratory symptoms, such as shortness of breath, chest tightness, wheezes, and cough, together with variable expiratory airflow limitation. Airflow limitation may later become persistent (GINA, 2023). The diagnosis of asthma is based on the history of these characteristic symptoms and evidence of variable expiratory airflow limitation from bronchodilator reversibility testing or other tests (GINA, 2023).
Interventions
DIAGNOSTIC_TESTLong non-coding MALAT1 gene expression assay:
RNA extraction (isolation from whole blood) II-Synthesis of CDNA from RNA by reverse transcription III-Gene expression assay by RT-PCR reaction
Sponsors
Sohag University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
15 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* All patients were admitted to Sohag University Hospital, chest department DIAGNOSED AS BRONCHIAL ASTHMA
Exclusion criteria
* Patients with malignancies, major trauma or surgery, and acute or chronic infectious diseases were excluded.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Long non-coding MALAT1 gene expression assay: | 2 MONTHS | Gene expression assay by RT-PCR reaction |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| TNF-alpha | 2 MONTHS | measured in serum by ELISA |
Countries
Egypt
Outcome results
None listed