Endothelial Dysfunction, Malnutrition, Calorie, Mood Disorders
Conditions
Keywords
relative energy deficiency, gut permeability, rowers
Brief summary
This study aims to learn about relative energy deficiency in male and female rowers at the end of the direct sport preparation phase of the annual training cycle. The main questions: \- How will energy intake influence leptin, cortisol levels, mood, gut discomfort, and permeability? The observational study involves male and female rowers from the Polish rowing team. Participants will perform 2000 meter ergometer test and 6000 ergometer test in annual training cycle. Researchers will compare outcomes from both tests.
Interventions
Rowers will perform 2000m ergometer test. A 2,000-meter trial is a standard test used to assess performance in rowers.
Sponsors
Poznan University of Physical Education
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 23 Years
Healthy volunteers
No
Inclusion criteria
* Membership in Polish Rowing Team, * minimum five years of training, * total training time minimum of 240 minutes, * filling out a food diary, * finishing 2000-meter ergometer test.
Exclusion criteria
* Probiotics within the last three months, * prebiotics within the last three months * antibiotic therapy within the last three months, * dietary regime, * gastrointestinal diseases.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| -FABP (intestinal fatty acid binding protein)to measure epithelial wall injury | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period | I-FABP concentration measured in blood \[ng/ml\] using commercially availableenzyme-linked immunosorbent assays |
| Leptin to measure long-term energy balance | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period | Leptin concentration measured in blood \[ng/ml\] using commercially availableenzyme-linked immunosorbent assays |
| Cortisol to measure stress | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period | Stress hormone concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| sCD14 multifunctional lipopolysaccharide receptor | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period | sCD14 concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays |
| Claudin to measure tight junction leakage | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period | Claudin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| POMS | day before the test | Profile of Mood States |
| REST-Q sport | day before the test | Recovery-Stress questionnaire for Athletes |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Total body water | in the morning before the test up to 1 hour after getting up | Body water \[%\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) |
| Lean body mass | in the morning before the test up to 1 hour after getting up | Lean body mass \[kg\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) |
| energy | Time Frame: whole day (24hours) before test, in the morning before the test | energy \[kcal \]of food intake measured by food diary |
| Self reported scale of gastrointestinal symptoms e.g.burping, heartburn, flatulence, abdominal pain, the urge to reiterate, vomiting, diarrhoea, nausea, dizziness, and stitch | up to 1 hours after the test | 10-point scale where 10 was the symptom with the greatest severity |
| Height | in the morning before the test up to 1 hour after getting up | \[cm\] measured by high meter |
| protein | Time Frame: whole day (24hours) before test, in the morning before the test | protein \[g \]of food intake measured by food diary |
| fat | Time Frame: whole day (24hours) before test, in the morning before the test | fat \[g \]of food intake measured by food diary |
| carbohydrate | Time Frame: whole day (24hours) before test, in the morning before the test | carbohydrate \[g \]of food intake measured by food diary |
| fiber | Time Frame: whole day (24hours) before test, in the morning before the test | fiber \[g \]of food intake measured by food diary |
| Body mass | in the morning before the test up to 1 hour after getting up | Body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) |
| Body fat | in the morning before the test up to 1 hour after getting up | Body fat \[%\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) |
Countries
Poland
Contacts
Primary ContactHanna Dziewiecka, Msc
Backup ContactAnna Skarpańska-Stejnborn, Profesor
Outcome results
None listed