Muscle Protein Synthesis
Conditions
Brief summary
Rationale: Collagen protein is the central structural component of extracellular connective tissues within skeletal muscle, bone, cartilage and skin. Dietary collagen peptides are a promising protein source to deliver the specific amino acid precursors required to support an increase in connective tissue protein synthesis across several tissues (e.g. muscle, skin). However, the digestion and absorption kinetics of multiple boluses of collagen peptides and the subsequent impact on muscle and skin connective tissue protein synthesis rates have not yet been assessed in vivo in humans. Objective: To assess the impact of ingestion of multiple boluses of collagen peptides on muscle connective and skin protein synthesis in vivo in humans. Study design: Double-blind, parallel-group, placebo-controlled intervention study. Study population: 20 healthy young males, aged 18-35 years. Intervention : Participants will perform unilateral resistance exercise followed by the ingestion of either 100 g of collagen peptides (in boluses) or a non-caloric placebo (flavoured water) drinks, while all drinks will contain vitamin C. Continuous intravenous stable isotope amino acid tracer infusions will be applied, plasma, skin and muscle samples will be collected in order to assess protein synthesis rates in skin and muscle tissue. Main study parameters/endpoints: Primary study parameters are muscle connective protein synthesis rates. Secondary study parameters are skin and myofibrillar protein synthesis rates, plasma amino acid concentrations and body composition.
Interventions
DIETARY_SUPPLEMENTCollagen protein
Ingestion of 40, 20, 20 and 20 gram of collagen protein right after and every 2 hours after a bout of exercise
BEHAVIORALResistance exercise
A single resistance exercise session of the leg press and leg extension exercise
Sponsors
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Intervention model description
Double-blind, parallel-group, placebo-controlled intervention study
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Aged between 18-35 years * Male * Healthy, recreationally active (participating in recreational sports activities ≥ 1 and ≤ 6 h per week, with a maximum of 2 h resistance-type exercise) * 18.5 ≤ BMI ≤ 30 kg/m2 * No physical limitations (i.e. able to perform all activities associated with daily living in an independent manner).
Exclusion criteria
* Female * Smoking * Musculoskeletal disorders * Metabolic disorders * Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications). * Chronic use of gastric acid suppressing medication or anti-coagulants * Unstable weight over the last three months * Diagnosed GI tract disorders or diseases * Blood donation in the past 2 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle connective protein synthesis rates rested leg | one value calculated over 8 hours | The primary analysis will be an independent t-test, comparing postprandial muscle connective protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the rested condition between groups. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscle connective protein synthesis rates exercised leg | one value calculated over 8 hours | Independent t-test, comparing postprandial muscle connective protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the exercised condition between groups. |
| Myofibrillar protein synthesis rates rested leg | one value calculated over 8 hours | Independent t-test, comparing postprandial myofibrillar protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the rested condition between groups. |
| Myofibrillar protein synthesis rates exercised leg | one value calculated over 8 hours | Independent t-test, comparing postprandial myofibrillar protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the exercised condition between groups. |
| Skin protein synthesis rates | one value calculated over 8 hours | Independent t-test, comparing postprandial skin protein synthesis rates over the 0-8 h period (i.e., one integrated value). |
| Plasma insulin concentrations | measured over the 8 hour post-prandial period | Plasma insulin concentrations |
| Plasma amino acids concentrations | measured over the 8 hour post-prandial period | Plasma amino acids concentrations |
Other
| Measure | Time frame | Description |
|---|---|---|
| Dietary macronutrient intake | 2 days before test days | assessed by written dietary intake records |
| Body mass | baseline | body mass in kg |
| Height | baseline | Height in m |
| Lean mass | baseline | Measured by DXA |
| One repetition maximum of the legs | baseline | maximum strength of both legs |
| Age | baseline | age in years |
Countries
Netherlands
Contacts
Primary ContactLuc JC van Loon, PhD
Outcome results
None listed