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Protein Requirements Amongst Male Masters-Level Athletes Following a Cycling Exercise Bout as Determined by the Indicator Amino Acid Oxidation Technique

Protein Requirements Amongst Male Masters-Level Athletes Following a Cycling Exercise Bout as Determined by the Indicator Amino Acid Oxidation Technique

Status
Recruiting
Phases
Unknown
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT06431334
Enrollment
8
Registered
2024-05-28
Start date
2024-06-21
Completion date
2026-06-01
Last updated
2026-02-09

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Dietary Reference Intakes, Healthy, Increased Metabolic Requirements

Brief summary

Masters level cyclists are a population above the age of 35 years who frequently participate in prolonged as well as heavy-volume training. Like most endurance-trained athletes, a greater recommended dietary allowance (RDA) for protein of 1.2-1.4 g/kg/bw is suggested. Dietary protein intake is vital for maximizing the benefits of training and ensuring optimal recovery. Dietary recommendations traditionally have been determined through nitrogen balance techniques, however, recent research indicates how this method is potentially underestimating protein requirements. Therefore, there is a need to reassess current dietary recommendations in order to meet the demands of physical activity for highly active populations. Recent efforts to understand protein requirements during rest and following exercise have been completed using the indicator amino acid technique (IAAO). This non-invasive method is reported to provide a robust measure of protein requirements. However, there is limited work in older (≥60 years) active populations. The purpose of this study is to measure the protein requirements in master cyclists, following an endurance training session, using the non-invasive IAAO technique.

Interventions

DIETARY_SUPPLEMENTAmino Acid Intake

Amino acid intakes will vary between 0.2 to 2.8 g/kg/d

Sponsors

McGill University
Lead SponsorOTHER

Study design

Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE

Eligibility

Sex/Gender
MALE
Age
65 Years to No maximum
Healthy volunteers
Yes

Inclusion criteria

* 18.5\< BMI \<35 kg/m\^2 * Overtly healthy masters level cyclists ≥65 years * Masters level cyclist training (\>4 days per week; \~100 km per week) * Body mass stable over previous 6-months * Availability for multiple metabolic trials (\~7 trials) * Ability to travel to and from laboratory facility

Exclusion criteria

* Recent history of weight loss or weight gain (\>5% body weight) * Uncontrolled hypertension * ≥2 chronic diseases (e.g. diabetes, osteoporosis, dyslipidemia) * Acute illness that could affect protein metabolism (HIV, renal dysfunction, influenza) * Currently using anti-inflammatory medications * Inability to adhere to study protocol (i.e., alcohol, caffeine, dietary restrictions) * Regular tobacco user * Illicit drug use * Habitually ingesting ≥3 g/kg/bw

Design outcomes

Primary

MeasureTime frameDescription
13CO2 Excretion7-weeksMeasured by continuous-flow isotope ratio mass spectrometry

Secondary

MeasureTime frameDescription
L[13C]phenylalanine Oxidation7-weeksMeasured by gas chromatography-mass spectrometry

Countries

Canada

Contacts

CONTACTTyler A. Churchward-Venne, Ph.D.
tyler.churchward-venne@mcgill.ca(514) 398-4184
CONTACTDamian Papadopoulos, B.Sc
damian.papadopoulos@mail.mcgill.ca(514) 561-7860

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 10, 2026