Cholesteatoma, Microbial Colonization, Otitis Media, Suppurative Chronic
Conditions
Keywords
Chronic suppurative otitis media, Cholesteatoma, Ear discharge
Brief summary
This study is planned to reveal the microbiological agents and drug sensitivities to these agents in patients diagnosed with chronic suppurative otitis media who complain of ear discharge.
Detailed description
Patients with complaints of ear discharge and who were diagnosed with chronic suppurative otitis media will be included in the study. Patients will be divided into two groups: CSOM with cholesteatoma and CSOM without cholesteatoma. One hundred ten patients from each group will be included in the study. After a comprehensive medical history is taken for each patient, the patient's external auditory canal will be cleaned with sterile aspirators. A sterile culture will be taken from the discharge of the ear and sent to microbiology. With the data obtained from microbiological examination, pathogens will be classified as gram-positive, gram-negative, anaerobic, and fungal and examined according to monomicrobial and polymicrobial growth. Antibiotic susceptibility tests will be conducted to determine the effectiveness of antibiotics against the bacteria causing the infection.
Interventions
DIAGNOSTIC_TESTMicrobiological culture
Samples taken from chronic suppurative otitis media patients in the Medical Microbiology Laboratory of Selçuk University Faculty of Medicine will be planted in 5% sheep blood Columbia agar, eosin methylene blue agar and saboraud dextrose agar, and the media will be incubated at 37oC for 24-48 hours. Growing bacteria and fungi were examined using traditional methods \[Gram staining, catalase test, coagulase test, bacitracin disk, PYR test (L-pyrrolidonyl-β-naphthylamide), oxidase test, IMViC test, lactophenol cotton blue, etc.\] and VITEK 2 (bioMerieux, France).
Sponsors
Selcuk University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
No
Inclusion criteria
1. Patients diagnosed with chronic suppurative otitis media 2. Patients over 18 years of age
Exclusion criteria
1. Chronic nonsuppurative otitis media patients 2. Patients diagnosed with external ear canal/middle ear malignancy 3. Patients who take immunosuppressive drugs 4. Immunosuppressive patients 5. Patients who used antibiotics (topical-systemic) in the last week
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Microbiology of chronic suppurative otitis media | 2024-2026 (24 months) | Microbiology in patients diagnosed with chronic suppurative otitis media with and without cholesteatoma |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Antibiotic susceptibility in chronic suppurative otitis media | 2024-2026 (24 months) | Investigation of antibiotic susceptibility and resistance in patients diagnosed with chronic suppurative otitis media with and without cholesteatoma. |
Countries
Turkey (Türkiye)
Contacts
Primary ContactBurcu Tuğba Aldora
Outcome results
None listed