Inflammatory Bowel Diseases, Spondyloarthritis
Conditions
Brief summary
This project aims to develop and validate a model of human organoids derived from patients with Spondyloarthritis, focusing on synovial and intestinal tissues as targets of the gut-joint axis. The tissue marker profile of patient-derived organoids studied by gene expression, immunohistochemistry, and cytokine production profile will be compared with that of controls in order to test for the presence of specific biomarkers.
Interventions
PROCEDURESynovial ultrasound-guided biopsy Intestinal biopsy
Patients will undergo biopsies if needed for standard-of-care follow-up and after clinical indication.
Sponsors
Fondazione Policlinico Universitario Agostino Gemelli IRCCS
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Inclusion criteria
* More than 18 years of age; * Ability to understand and sign an informed consent form; * Clinical indication for intestinal and/or synovial biopsy; * Only for SpA patients group: meeting ASAS classification criteria.
Exclusion criteria
* Actively treated cancer; * Severe comorbidities that, in the investigator's opinion, may affect the quality of samples for planned experimental applications; * Only for patients undergoing ileal-colic biopsy: history of colorectal cancer disease, celiac disease; * Only for patients undergoing a synovial biopsy: septic arthritis.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Viability assessment | 24 months | The viability of organoids will be determined using an adenosine triphosphate (ATP) detection assay based on luminescence using a commercially available luciferase. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Production of pro-inflammatory cytokines | 24 months | The production of cytokines in the culture supernatant of organoids will be evaluated using enzyme-linked immunosorbent assay (ELISA) |
Other
| Measure | Time frame | Description |
|---|---|---|
| Invasion capacity assessment (synovial organoids) | 24 months | Fibroblast-like synoviocytes (FLS) will be dispensed into a well of a Fluorobook invasion plate. The fluorescence will be measured as an index of FLS invasion capacity in the presence of a basement membrane extract. |
Countries
Italy
Contacts
Primary ContactMaria Antonietta D'Agostino, Professor
Backup ContactGerlando Natalello, Dr
Outcome results
None listed