Phenotypic and Genotypic Characteristics of Pseudomonas Aeruginosa Isolates in Sohag University Hospitals

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT06417593

Enrollment

100

Registered

2024-05-16

Start date

2024-04-01

Completion date

2025-03-01

Last updated

2024-05-20

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Pseudomonas Aeruginosa Infection

Brief summary

Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions from Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections that will be obtained under complete aseptic precautions.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

1 Years to 80 Years

Healthy volunteers

Inclusion criteria

* Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.

Exclusion criteria

* Any infection before 48 hours of patient admission at Hospital

Design outcomes

Primary

Measure	Time frame	Description
Isolation and identification of pseudomonas aeruginosa causing different nosocomial infections.	6 months	Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions
Identification of antibiotic susceptibility pattern of isolated strains of pseudomonas	6 months	Antibiotic susceptibility testing of the isolates will be performed using disc diffusion method according to clinical laboratory standards institute (CLSI) guidelines
Detection of biofilm forming isolates	6 months	Detection of biofilm forming isolates by tissue culture plate method.
Detection of virulence and antibiotic resistance genes of pseudomonas aeruginosa.	6 months	Detection of virulence and antibiotic resistance genes by conventional Polymerase Chain Reaction

Countries

Egypt

Contacts

Primary ContactMartina Gamil Fayez, Demonstrator at Microbiology

martina.gameel@med.sohag.edu.eg01272602453

Outcome results