Patients Undergoing Esophagectomy, Patients With Abdominal Infection, Patients With Severe Neurological Lesion, Patients With Severe Polytrauma
Conditions
Brief summary
Patients admitted for polytrauma, severe neurological injury, post-operative monitoring or sepsis/septic shock present with significant inflammation, leading to immunoparalysis, which is responsible for infection, particularly prolonged infection. A study of their lymphocyte phenotype over time could help explore the phenomenon of immunoparalysis.
Interventions
PROCEDURELymphocyte Phenotyping Procedure
For all patients, lymphocyte phenotyping will be performed on biological samples taken on D0, D7 and the day of discharge. In case of infection, phenotyping will also be performed on the day of infection diagnosis and on the day corresponding to half the duration of antibiotic therapy. For phenotyping, only 1.5 mL will be used. In case of infection, an additional 1mL will be collected (0.5mL on the day of inclusion diagnosis and 0.5 mL on the day corresponding to half the duration of antibiotic therapy). Blood is collected during sampling, which takes place several times a day as part of the standard management of these patients. It is important to note that phenotyping is not routinely performed as part of routine care. An additional tube is therefore taken specifically for this analysis, but this is done without the need for an additional puncture.
Sponsors
University Hospital, Brest
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
For all patients, lymphocyte phenotyping will be performed on biological samples taken on D0, D7 and the day of discharge. In case of infection, phenotyping will also be performed on the day of infection diagnosis and on the day corresponding to half the duration of antibiotic therapy. For phenotyping, only 1.5 mL (0.5 mL per analysis) will be used (if no infection). In case of infection, an additional 1mL will be collected (0.5mL on the day of inclusion diagnosis and 0.5 mL on the day corresponding to half the duration of antibiotic therapy). Blood is collected during sampling, which takes place several times a day as part of the standard management of these patients.
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Postoperative esophagectomy surveillance within the last 24 hours. * Patients with intra-abdominal infection * Neurological patients with intubation planned for more than 24 hours (subarachnoid hemorrhage with coma, severe head trauma, ischemic or hemorrhagic stroke with coma). * Polytrauma with intubation planned for more than 24 hours * Patient aged 18 and over * Patient with social security coverage Non-inclusion criteria : * Hereditary immune deficiency HIV-AIDS * Malignant hemopathies * Immunosuppressive treatment other than corticosteroid therapy or chemotherapy * Patient under legal protection * Pregnant or breast-feeding women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Lymphocytes numbers variation | Day 0 | \- Numbers of various lymphocytes (LT, LB, NK and their subclasses) at D0 according to reason for admission (postoperative vs sepsis/septic shock vs polytrauma vs neurological injury) |
| Lymphocytes percentages variation | Day 0 | \- Percentage rate of various lymphocytes (LT, LB, NK and their subclasses) at D0 according to reason for admission (postoperative vs sepsis/septic shock vs polytrauma vs neurological injury) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Lymphocytes percentages variation in case of infection | Day 0 | Percentage rate in various lymphocytes (LT, LB, NK and their subclasses) at D0 |
| Lymphocytes numbers variation in case of infection | Day 0 | Numbers rate in various lymphocytes (LT, LB, NK and their subclasses) at D0 |
Countries
France
Outcome results
None listed