Alveolar Bone Loss, Alveolar Bone Resorption, Vitamin D Deficiency
Conditions
Keywords
Dental Implants, Alveolar Bone Loss, Alveolar Process
Brief summary
The aims of the study was to correlate the extent of the dimensional alveolar bone changes after tooth extraction and contextual guided bone regeneration with serum vit D levels in 14 patients. Moreover, at 4 months, a small bone sample was collected in order to correlate histological and immunohistochemical parameters of bone with vit D serum levels.
Detailed description
The study included 14 patients requiring tooth extraction and subsequent post-extraction bone regeneration with heterologous graft material of porcine origin. Four months after extraction, dental implants were placed, and insertion torque values (ITV) and implant stability quotients (ISQ) were recorded. During implant placement, a small bone sample was collected for histological analysis. The aim of the study was to analyze the correlation between serum vitamin D levels and post-extraction bone volume contraction, collagen type I (Col1A1), osteocalcin, osteopontin, runt-related transcription factor 2 (Runx2), ITV, ISQ, Newly Formed Bone Tissue (NFBT).
Interventions
PROCEDURETooth extraction, guided bone regeneration and dental implant
The arm received tooth extraction and contextual guided bone regeneration. After 4 months, a dental implant was inserted.
Sponsors
Studio Odontoiatrico Associato Dr. P. Cicchese e L. Canullo
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Male or female adult (≥ 18 years old) * Patients willing to participate and to attend the planned follow-up visits
Exclusion criteria
* Absence of Type 1-Medical conditions requiring prolonged use of steroids and/or medications that can interfere with bone metabolism * History of leukocyte dysfunction and deficiencies * History of neoplastic disease requiring the use of radiation or chemotherapy * History of renal failure * Metabolic disorders such as osteoporosis and correlated vit D or antiresorptive medications assumption * Physical handicaps that would interfere with the ability to perform adequate oral hygiene * History of uncontrolled endocrine disorders * Alcoholism or any drug abuse * History of immunodeficiency syndromes * Smoker of 10 cig per day, cigar equivalents or tobacco chewers * Conditions or circumstances which in the opinion of the investigator would prevent completion of study participation or interfere with the analysis of the study results, such as history of non-compliance or unreliability * Mucosa disease such as erosive lichen planus * Residual post-extraction site with intact bone walls * History of local irradiation therapy * Persistent intraoral infection
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Dimensional alveolar bone changes | From extraction to implant insertion (4 months) | Volumetrical changes of alveolar bone after tooth extraction measured by superimposition of two intraoral scans. The first was registered before the extraction of the tooth. The second was registered after 4 months (before inserting implant). The parameter used to measure the dimensional alveolar bone change is called Integrated Distance and it is expressed in mm3. |
| Evaluation of new formed bone tissue | Time of implant insertion: 4 months after extraction | Evaluation of new formed bone tissue performed by histomorphometric methods. |
| Evaluation of Total Calcified Tissue (TCT) | Time of implant insertion: 4 months after extraction | Evaluation of Total Calcified Tissue composed by the residual bone substitute utilized for regeneration plus the NFBT. The evaluation was performed by histomorphometrics methods. |
| Expression of Collagen A1 | Time of implant insertion: 4 months after extraction | quantitative analysis of immunohistochemistry performed using deconvolution tool for ImageJ. |
| Expression of Osteocalcin | Time of implant insertion: 4 months after extraction | quantitative analysis of immunohistochemistry performed using deconvolution tool for ImageJ. |
| Expression of Osteopontin | Time of implant insertion: 4 months after extraction | quantitative analysis of immunohistochemistry performed using deconvolution tool for ImageJ. |
| Expression of Runx-2 | Time of implant insertion: 4 months after extraction | quantitative analysis of immunohistochemistry performed using deconvolution tool for ImageJ. |
| Evaluation of Implant Stability Quotient (ISQ) | Time of implant insertion: 4 months after extraction | Evaluation of Implant Stability Quotient after the implant insertion. The measurement was performed with Osstell system (W&H) |
| Evaluation of Implant Torque Value (ITV) | Time of implant insertion: 4 months after extraction | Evaluation of Implant Torque Value at the implant insertion performed with the surgery implant device. |
Countries
Italy
Outcome results
None listed