Age Related Differences in Respiratory Immune Responses in Influenza Virus Infection

Respiratory Immune Responses in Human Influenza Virus Infection Across Age to Understand What Dictates Disease Severity

Status

Enrolling by invitation

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT06401720

Acronym

NPA-DC

Enrollment

800

Registered

2024-05-07

Start date

2024-01-01

Completion date

2030-12-31

Last updated

2024-05-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Influenza A Infection, Influenza B Virus Infection, RSV Infection, SARS CoV 2 Infection

Keywords

Influenza A virus (IAV), Influenza B virus (IBV), SARS CoV 2, respiratory syncytial virus (RSV), immunology, age-related differences

Brief summary

The goal of this observational study is to understand immune responses to viral airway infection in adults, including the elderly. The main question(s) to answer is/are: Why do some individuals acquire only asymptomatic or mild Influenza A virus (IAV) infection while others become severely ill and even succumb to the same disease? Participants will be asked to donate samples when seeking health care for influenza-like symptoms or if hospitalized for IAV or SARS-CoV-2. Samples asked for are: * Blood sample by venepuncture * Blood sample by capillary sampling * Nasopharyngeal aspirate * Nasopharyngeal swab * Endotracheal tube aspirate * Nasal swab * Nasal curette * Breath Explor (sampling of expired air) Researchers will compare obtained results with the same type of samples from healthy controls.

Detailed description

This observational study will specifically investigate different immunological parameters in blood and at the site of infection in patients with IAV symptoms or confirmed infection to understand parameters related to disease course. Blood and airway samples will be analyzed for immune cell subsets and their functionality, antibodies and other soluble mediators and linked to clinical data. Immune cells will be analyzed using multi-color flow cytometry, RNA sequencing, and in vitro functionality assays. Fluids (plasma, serum, supernatants from airway samples and cell culture supernatants) will be analyzed using ELISA (and or similar methods), proximity extension assay (Olink), etc.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

Patients: * Influenza A like symptoms and/or * Confirmed airway infection

Exclusion criteria

Patients: * Current malignancies * Immunosuppressive treatment, not including/except hydrocortisone

Design outcomes

Primary

Measure	Time frame	Description
Frequency of immune cells	Samples are taken at several different occasions when the patient is experiencing an airway infection and during convalescence. Samples are taken during a time frame of up to 6 months.	Flow cytometric analysis of white blood cell phenotype and frequency in PBMC, nasopharyngeal aspirate, nasopharyngeal swab, nasal swab, nasal curette, and endotracheal tube aspirate samples.

Countries

Sweden

Outcome results