Diabetes Type 2
Conditions
Keywords
exercise, blood glucose oxidative stress, diabetes mellitus
Brief summary
Diabetes is a prevalent metabolic disease that leads to increased blood sugar levels due to insulin shortage or resistance. Type 2 diabetes is often linked to obesity, which can increase insulin resistance. Meteorin-like protein (METRNL) is a new secreted protein that affects insulin sensitivity and has been found to be negatively related to serum glucose levels in people with diabetes. Oxidative stress in diabetics can stimulate the production of inflammatory mediators, increasing the activity of the antioxidant system. Aloe vera, a widely used plant, has been used for treating diabetes, wound healing, tumors, and inflammatory bowel disease. It has anti-inflammatory, antioxidant, neuroprotective, antidepressant, and memory-enhancing effects. Exercise can improve diabetic patients' conditions by increasing the expression of GLUT4 protein and skeletal muscle. High-intensity interval training has been shown to increase the expression of the METORIN gene in human subjects and decrease MDA in type 2 diabetic patients. However, no studies have examined the simultaneous effect of exercise and Aloe vera on diabetes indicators. The present study aims to investigate the response of METRNL and some oxidative and antioxidant plasma indicators to high-intensity interval training and Aloe vera supplementation in type 2 diabetic men. The findings indicate that exercise and consumption of plants with anti-inflammatory and antioxidant properties can be effective in modulating the pathological effects of diabetes and increasing insulin sensitivity.
Interventions
BEHAVIORALExercise
The HIIT group underwent intervention with high intensity interval training. The second group was treated only with aloe vera supplementation for 12 weeks. A third group underwent a combination of HIIT and supplementation, while a fourth - CON group kept usual activities during the intervention duratino
DIETARY_SUPPLEMENTAloe vera
Aloe Vera group was treated only with aloe vera supplementation for 12 weeks.
Sponsors
University of Nis
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Outcomes Assessor)
Masking description
The participants were blinded for the supplementation.
Eligibility
Sex/Gender
MALE
Age
25 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* Male participants who have been diagnosed with T2DM at least 3 months prior to the start of the study according to standard criteria * Inactive participants according to the International Physical Activity Questionnaire (IPAQ) * Participants receiving stable medical therapy for T2DM, excluding those on exogenous insulin therapy * Participants who were free from injuries or chronic health conditions that could limit their ability to safely participate in an intense exercise program were included in the study
Exclusion criteria
* Fasting glucose above 270 mg / dL * Exogenous insulin * History of diabetic complications (the final stage of proliferative diabetic retinopathy, or subsequent overt nephropathy, diabetic ketoacidosis, or severe diabetic neuropathy) * Hereditary glucose and galactose malabsorption
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| METRNL - Meteorin Like, Glial Cell Differentiation Regulator | Baseline and 12 weeks | Plasma METRNL content was measured using a special kit (Mybiosource company, USA) with a sensitivity of \<0.64 ng / ml, range of 1.56-100 ng / ml by ELISA method |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Glutathione peroxidase (GPx) | Baseline and 12 weeks | GPx was measured using a special kit (abcam company, USA) through ELISA method. |
| Serum superoxide dismutase (SOD) | Baseline and and 12 weeks | SOD was measured using a special kit (Abcam company, USA) through ELISA method. |
| Total antioxidant blood capacity (TAD) | Baseline and 12 weeks | TAD was measured using a special kit (Abcam company, USA) through ELISA method. |
| Plasma malondialdehyde (MDA) | Baseline and 12 weeks | MDA was measured using a special kit (Abcam company, USA) through ELISA method. |
Countries
Iran
Outcome results
None listed