Endothelial Dysfunction
Conditions
Keywords
endotoxin, gastrointestinal symptoms, gut permeability
Brief summary
The study aimed to check the 2000m ergometer test on markers of gut permeability in the competitive phase of rowers. 18 members of the Polish rowing team took part in the study.
Detailed description
The phenomenon of epithelial wall leakage has been the subject of investigations within athletic domains such as running and cycling. Nevertheless, there exists an insufficiency of understanding regarding gut permeability in alternative disciplines, such as rowing, particularly following the completion of a standard competitive distance of 2000 meters. Hence, the principal objective of our study was to assess gut permeability after completing a 2000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 2000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1-hour of restitution. Parameters, such as I-FABP, LPS, LBP, and zonulin, were determined using appropriate biochemical tests.
Interventions
OTHERergometer test
2000meter rowing ergometer test
Sponsors
Poznan University of Physical Education
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
18 Years to 23 Years
Healthy volunteers
Yes
Inclusion criteria
* minimum five years of training, * five training sessions per week minimum, * total training time minimum of 240 minutes, * membership in the Polish Rowing Team, * finishing the 2000-meter ergometer test.
Exclusion criteria
* antibiotic therapy within the last three months, * probiotics and prebiotics within the last three months, * dietary regime, * gastrointestinal diseases.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| zonulin to measure tight junction leakage | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| LBP (lipopolysaccharide binding protein) to measure endotoxin | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays |
| LPS (lipopolysaccharide) to measure endotoxin | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | LPS concentration measured in blood \[EU/Ll\] using commercially available enzyme-linked immunosorbent assays |
| Lactic acid to measure fatigue after the race | Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test. | Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Body composition - water | in the morning before the test | % of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
| carbohydrate | day before test | carbohydrate \[g\] intake day before the test measured by 24h dietary recall |
| Lean body mass | in the morning before the test | Lean body mass \[kg\] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
| Body composition - fat | in the morning before the test | % of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
| fiber intake | day before test | fiber \[g\] intake day before the test measured by 24h dietary recall |
| protein | day before test | protein \[g\] intake day before the test measured by 24h dietary recall |
| energy | day before test | energy \[kcal \]of food intake measured by 24h dietary recall |
| body mass | in the morning before the test | Body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
Countries
Poland
Outcome results
None listed