Atherosclerosis and Calcific Aortic Stenosis with and Without Diabetes
Conditions
Brief summary
The project aims to shed light on the role of Sirtuins, enzymes belonging to the third class of histone deacetylases (class III) involved in epigenetic modifications (deacetylation), focusing mainly on Sirtuin 6 (SIRT6), as a regulator of atherosclerosis and severe aortic valve stenosis through molecular-based and epigenetic studies in human VSMC cells.
Interventions
DIAGNOSTIC_TESTlncRNA signature analyses
Specific validation experiments will be performed by Real-Time PCR (C-reactive protein), Western Blot, flow cytometry and microscopy
Sponsors
Federico II University
IRCCS SYNLAB SDN
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Patients with atherosclerosis or calcific aortic stenosis with and without diabetes older than 18 years of either sex; * Patients without atherosclerosis undergoing surgical procedure for endocarditis; * Patients who will have signed informed consent
Exclusion criteria
* Patients for whom biological material cannot be recovered for study purposes; * Patients who refuse to participate in the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To evaluate by analysis of data obtained from NGS techniques whether and which proteins belonging to the Sirtuin family may be deregulated in patients with atherosclerosis or calcific aortic stenosis | 36 months |
Secondary
| Measure | Time frame |
|---|---|
| To evaluate the experimental results achieved in the clinical-diagnostic context | 36 months |
Countries
Italy
Outcome results
None listed