Ageing
Conditions
Brief summary
The main goal of this study is to determine the metabolic availability of methionine in black beans, lysine in sorghum and lysine in milk using the indicator amino acid oxidation method both in older and in younger men.
Interventions
OTHERBlack beans
Black beans cooked and mashed and amino acid mixture as liquid drink to make methionine the limiting amino acid in the food consumed
OTHERMilk
Skimmed milk as liquid drink and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed
OTHERSorghum
Sorghum cooked and mashed and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed
Sponsors
Wageningen University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Intervention model description
It is a parallel intervention study. The intervention does not involve a drug or biologic product, however does involve an adaptation diet and a diet with measured amino acids dissolved in water, during the test day. We investigate the amino acid metabolic availability using the IAAO method.
Eligibility
Sex/Gender
MALE
Age
20 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
Gender: Male Age: 20-35 years or 65-80 years Body Mass Index (BMI): 18.5 - 30.0 kg/m2 Healthy as assessed with a questionnaire Regular and normal eating habits as assessed with a Food Frequency Questionnaire (FFQ) Willing to comply with study procedures and use of data Having given written informed consent
Exclusion criteria
Chronic disease, for example: * Diabetes mellitus / being treated for high blood glucose * Severe cardiovascular disease (exception: hypertension) * Hepatic disease (e.g. hepatitis) * Renal disease * Cancer * Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding) * Pancreatitis History of medical or surgical events that may affect GI function, and the study outcomes or disease risk for participants, for example: * Bariatric surgery * Gastrointestinal tract surgery * Digestive tract disorder * Chewing problems * History of deep venous thrombosis without anti-coagulation medication Medicine use that interferes with, protein metabolism, GI function and the study outcomes, for example: * Glucose lowering drugs * Proton pump inhibitors * Laxatives Habits that interfere with the study outcomes: * Protein supplement use (current use or less than 2 weeks past use) * Smoking * Drug use * Alcohol consumption \>21 units/week and/or \>4/day * Following a weight-loss diet, medically prescribed diet or other diet with a low calorie intake or an unbalanced nutrient intake like a vegan or very low carbohydrate diet * Moderate to high intense physical activity for more than 5 hours a week Other: * Self-reported allergy or intolerance to the tested products * Weight loss of more than 3 kg in the 3 months prior to study screening * Current participation in other research and \<2 months prior participation in other research * Not having a general practitioner * Not willing to accept information-transfer concerning participation in the study , or information regarding his health to his general practitioner * Working or doing an MSc- thesis at the department of Human Nutrition and Health at Wageningen University & Research
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method. | 1 measurement to be calculated from repeated measurements on several test days separated by 1 week | Indicator amino acid oxidation method is used to measure metabolic availability of the limiting amino acid by measuring the oxidation of the 13C-labelled indicator amino acid in expired breath as 13CO2 when giving different levels of the limiting amino acid. Methionine metabolic availability will be assessed in black beans and lysine metabolic availability in milk and sorghum. |
Countries
Netherlands
Outcome results
None listed