Damage Dna, Genotoxicity, Occupational Exposure
Conditions
Keywords
Biomonitoring, Genotoxic damage, Micro and nanoplastic
Brief summary
The biomonitoring study aims to evaluate genotoxic damage and other parameters of exposure in different occupational exposed populations that work directly with different plastics.
Detailed description
The exponential increase in the production and use of plastic goods translates into a parallel increase of environmental plastic waste that is continuously degraded into micro and nanoplastics (MNPLs). Information on their effects on human health is still preliminary, especially regarding human biomonitoring studies, thus more information is required for risk assessment evaluation. This observational study aims to evaluate the exposure to MNPLs in human populations occupationally exposed to different types of plastics (fibers and plastic waste). The study will evaluate the effect of exposure to MNPLs (genotoxic and microbiota effects) in different body samples (blood, exfoliated buccal cells, urine,and feces) and also will use those samples to evaluate the presence of MNPLs.
Interventions
OTHERMicro and nanoplastic
Biological samples will be obtained to evaluate the different biomarkers selected
Sponsors
Finnish Institute of Occupational Health
Ricard Marcos
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Aged over 18 years. * Healthy subjects defined by the absence of evidence of any active or chronic disease following a detailed medical and surgical history, a complete physical examination including vital signs, 12-lead ECG, haematology, blood chemistry, and urine analysis. * Able to participate and willing to give written informed consent and to comply with the study restrictions. * Have been working at the company for the last 48 months
Exclusion criteria
* Workers decline participation * Legal incapacity or inability to understand or comply with the requirements of the study. * Any hierarchical subordination to the PI * Clinically significant findings as determined by medical history taking, physical examination, ECG, and vital signs. * Submitted to X-ray in the three previous months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Characterization of macro and nanoplastics | Up to 1 year | The characterization of macro and nanoplastics will be done by Raman spectrosmicroscopy and FTIR (From exhaled breath condensate, blood and urine) |
| Genotoxic evaluation by the Micronucleus assay | Up to 1 year | Blood and buccal cells will be collected from donors to check for the presence of genotoxic permanent damage |
| Genotoxic evaluation by the Comet assay | Up to 1 year | Blood will be analysed to check for the presence of genotoxic primary damage |
| Cytokine release | Up to 1 year | Plasma will be analysed to check for the presence of cytokines |
| Microbiota analysis from feces by MinIOn | up to 1 year | Feces will be analysed to check for variations in microbiota in the exposed and control groups |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Analyses of surrogate biomarkers | Up to 1 year | Different metabolites will be analysed in the urine of participants, as an indirect measure to detect plastic exposure (p.eg. ftalates). The selection of the metabolite will depend of other analyses done on the project. |
Countries
Finland, Spain
Contacts
Primary ContactAlba Hernández, Professor
Outcome results
None listed