Cesarean Section Complications, Infertility Due to Nonimplantation, Infertility Secondary
Conditions
Brief summary
A prospective study that will take place in the hydrosonography clinic, comparing patients with an intact uterus to those who had a caesarean section. Eligible patients will preform a transvaginal ultrasound for evaluation of presence of a niche. After fixation of the transcervical catheter, we will flush 2 ml sterile NaCl 0.9% into the uterine cavity and aspirate the fluid. After collecting all samples, the samples will be snap frozen and stored at -80o C, until they undergo an immunological analysis.
Interventions
DIAGNOSTIC_TESTUterine lavage
Infusion of 2ml 0.9%NACL into the uterine cavity and aspiration of the fluid
Sponsors
Hadassah Medical Organization
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
\- Patients suffering from infertility and assigned for a hydrosonography exam for assessment of the integrity of the uterus and fallopian tubes.
Exclusion criteria
1. Patients with hydrosalpinx/pyosalpinx/sactosalpinx 2. Patients who went through surgical correction of a niche 3. Hormonal treatment- estrogens or progestins. 4. Immunosuppressive/immunomodulating medication
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Inflammatory cell population | Through study completion, an average of 1 year | The wash samples are processed, supernatant discarded and RBCs removed. Cells are then washed with FACS buffer ,subjected to antibody staining and examined by flow cytometry. Antibodies include: CD45,UV,CD3,CD19,CD66B,CD88, CD89, CD14,CD16,CD314,HLA-DR in order to identify monocytes, dendritic cells, T cells, B cells, NK and Neutrophil populations |
Countries
Israel
Contacts
Primary ContactChana Adler, MD
Outcome results
None listed