Healthy Volunteer Children
Conditions
Keywords
DNA damage, Chemical exposome, Hair polluants impregnations, Socio-economic characteristics
Brief summary
PESTIFOL will include children over 8 and under 12 years old of different socioeconomic positions (SEP), mostly living in the region of Pays de la Loire. This approach should enable us to assess the impact of the SEP, and therefore social inequalities, on chemical expositions and DNA damage, and to characterize which (classes/mixture of) pollutants are major source of DNA damage. The study will also address potential effects associated with geographical location on the exposome and its biological consequences.
Detailed description
Growing evidence points to a complex influence of the exposome on health outcomes. Epidemiological studies show associations between pesticides and cognitive delay and cancers. Only few were conducted in children. Chemical exposures differ between socioeconomic groups. The more disadvantaged the group, the higher are some exposures. However, the effect of the exposome on health is complicated to assess due to the long latencies of most diseases. It is therefore important to link social and chemical exposures to 'immediate' outcomes or molecular risk factors for human diseases. There is a strong connection between DNA damage and chronic diseases such as cancers. However, it is difficult to attribute these risks to specific impregnations. The present study will address the impact of socioeconomic position, hence social inequalities, on environmental exposures and their biological consequences in children. Our approach is to detect DNA damage in hair follicles and associate these measurements with pesticide detections in the hair matrix and socioeconomic position (SEP). PESTIFOL will include children over 8 and under 12 years old of different socioeconomic positions (SEP), mostly living in the region of Pays de la Loire. This approach should enable us to assess the impact of the SEP, and therefore social inequalities, on chemical expositions and DNA damage, and to characterize which (classes/mixture of) pollutants are major source of DNA damage. The study will also address potential effects associated with geographical location on the exposome and its biological consequences.
Interventions
OTHERHair cut
Hair locks and follicles will be cut twice in winter and spring
OTHERSocio-economic questionnaires
Information about parents education levels and professional occupation, geographic area, children gender, indoor and outdoor children's environmental exposures
Sponsors
Institut Cancerologie de l'Ouest
ANSES
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
HEALTH_SERVICES_RESEARCH
Masking
NONE
Intervention model description
Hair collection and parents questionnaires
Eligibility
Sex/Gender
ALL
Age
8 Years to 12 Years
Healthy volunteers
Yes
Inclusion criteria
* Children over 8 and under 12 years old at the time of inclusion * Children in school in class level CE2, CM1 or CM2 * Living in the Pays de La Loire region, at least 80% of the time * Written informed assent and consent obtained from the participant and both parents or legal representative prior to performing any protocol-related procedures.
Exclusion criteria
\- Disorder precluding understanding of trial information or informed assent.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Describe DNA damage | 4-6 months | Hair locks and hair follicles will be collected and will be analysed to evaluate the DNA damage : average amount of DNA break foci per nucleus cross-section |
| Describe socio-economic characteristics. | 4-6 months | The socio-economic characteristics will evaluated via parent's questionnaires : parents occupation and education level |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Characterize chemical exposome variations | 4-6 months | Chemical exposome: presence and amount of pollutant impregnations : total number of polluants detected in hair sample |
| Characterize socio-economic indicators | 4-6 months | The socio-economic characteristics will be evaluated via parents' questionnaires: parents' occupation and education level |
| Characterize socio-economic indicators, geographical area, seasons | 4-6 months | The socio-economic characteristics will be evaluated via parents' questionnaires: geographic area |
| Describe DNA damage | 4-6 months | Evaluate the DNA damage: average amount of DNA break foci per nucleus cross-section |
Countries
France
Outcome results
None listed