Adipose Tissue
Conditions
Keywords
Extracellular matrix compostion, Obesity, Age
Brief summary
The experiment is a simple cross-sectional study with three groups (n=10) in each group, young and old healthy men and obese young men. The study will investigate the composition of the adipose tissue extra cellular matrix. The main questions to answer, \- is there differences in the adipose tissue extracellular matrix in obese compared to young men is there differences in the adipose tissue extracellular matrix in old compared to young men
Detailed description
Three groups of young (n=10) and old (n=10) healthy men and obese young (n=10) men are recruited. After verbal and written information is provided signed consent is obtained. Subjects arrive at the lab overnight fasted and not having done exercise the prior day and having consumed a normal isocaloric carbohydrate rich diet. After a voiding and a 15 min rest body composition is determined by DXA. After this blood pressure is measured in the supine position in triplicate separated by 1 min. Following this a venous blood sample is obtained from a cubital vein, and kept on ice until further handling. Subsequently an adipose tissue biopsy is obtained by the Bergström technique applied with suction from an incision 3-5 cm lateral from the umbilicus. After compression and a 30 min rest, the subject perform an incremental exercise test to determine maximal fat oxidation. In brief pulmonary oxygen uptake and carbon dioxide excretion is measured using a COSMED Quark B2 on-line system during 5 min rest sitting on a cycle ergometer, and then during exercise with 3 min increments starting at 60 Watt and increasing by 35 watts. When the respiratory exchange ratio is above 1 the exercise is stopped. After a 5 min break an incremental test until voluntary exhaustion is performed to determine maximal oxygen uptake. This completes the experiment.
Interventions
Physiological testing, Sampling of blood and abdominal adipose tissue and respiratory measurements at rest and during exercise
Sponsors
University of Copenhagen
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Cross sectional study with three groups
Eligibility
Sex/Gender
MALE
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men aged \[18,35\] and BMI \[20,25\] * Obese men aged \[18,35\] and BMI \[30,40\] * Healthy older men aged \[60,75\] and BMI \[20,25\]
Exclusion criteria
* Present or former cardiovascular disease and chronical inflammatory disease * Consumption of medications and supplements that influence substrate use at rest and/or during exercise
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The effect of obesity and age on adipose tissue extracellular matrix composition | The human experimental days will be done over the first 6 months of 2024. After informed consent is obtained, each participant will be tested on one day. The laboratory tissue analysis will be performed second half of 2024. | Adipose tissue extracellular matrix composition will be measured in abdominal subcutaneous adipose tissue sampled 3-5 cm lateral to the umbilicus in 30 male subjects; hereof 10 young, 10 old and 10 young and obese. In brief the tissue will be delipidated and subsequently separated by DEAE chromotography (A weak anion exchange) and subsequently analysis of glycosaminoglycan disaccharide composition will be performed by MS. |
Countries
Denmark
Contacts
Primary ContactJørn Wulff W Helge, PhD
Backup ContactChristoffer Clemmensen, PhD
Outcome results
None listed