Aerobic Exercise, Metabolic Diseases
Conditions
Brief summary
Fitness is one of the best predictors for heart and brain disease. To increase ones fitness, the American Heart Association (AHA) says to exercise at least 150 minutes per week or 75 minutes per week if really hard. These exercise guides are pretty effective, however not everyone will get the same results. What individuals do outside of the exercise bout can influence the effectiveness of exercise. One of these factors is our time sitting, which has caused the phrase "sitting is the new smoking". Other studies have said that the metabolic benefits of exercise are decreased when you exercise after a few days of low activity (less than 5,000 steps per day). This is important in that exercise may not be able to fully offset these times of inactivity. However, these studies were only looking at different fats in the blood. As exercise increases fat burn up to 10 times in the muscle, more research is needed to understand how inactivity affects the muscle during exercise and after exercise. This study will help answer two questions: 1) How does a day of sitting a lot affect the muscle's ability to respond to exercise? and 2) How does a day of sitting a lot affect carbohydrate and fat burn during and after a bout of exercise? The investigators will answer these questions by having people complete one day of inactivity (less than 5,000 steps) or normal activity (more than 8,500 steps). Subjects will then come in the next day to bike somewhat hard for 1 hour. The investigators will take blood samples before, during, and after exercise to measure energy sources. The investigators will also collect pieces of skeletal muscle before and after exercise to see how the muscle responded to exercise. This study is significant for the publication of exercise guidelines to minimize risk of heart and metabolic diseases.
Interventions
OTHERInactivity
Low physical activity level prior to the exercise intervention.
Sponsors
Concordia University Wisconsin
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* free from acute or chronic illness (cardiac, pulmonary, liver, or kidney abnormalities, cancer, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other known metabolic disorders) * free from orthopedic limitations (including any artificial joints) no known lidocaine allergy * do not currently smoke or participate in other forms of tobacco use. * not currently in a structured exercise program
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Circulating lipid and carbohydrate metabolism | Baseline; 20 and 40, and 60 minutes into exercise; and every 30 minutes after exercise up to 4 hours | Investigate the role of sex and inactivity on lipid and carbohydrate metabolism following a bout of aerobic exercise. |
| Basal and post-exercise gene expression with inactivity | Baseline; 4 hours after exercise | Study the role of inactivity on basal and exercise-induced gene expression in skeletal muscle. |
Countries
United States
Outcome results
None listed