Bladder Cancer, Genomic Instability
Conditions
Keywords
Neoadjuvent chemotherapy, Molecular subtype, Bladder cancer, Genomic instability, Artificial intelligence
Brief summary
This study will allow the investigators to better assess the efficiency of neoadjuvant chemotherapy before cystectomy by training a predictive model on different patient cohorts with bladder cancer.
Detailed description
The project is based on three prospective cohorts of patients with MIBC: the VESPER trial (n=296), the St-Louis Hospital cohort (n=99), and the COBLAnCE cohort (n=312). Using WES and RNAseq, the investigators will determine genomic instability, DDR gene mutation and molecular subtypes. After digitization of tumour slides, the investigators will train and test predictive models based on deep learning approaches to predict outcome after neoadjuvant chemotherapy, either by estimating molecular subtypes and genetic features from pathological images, or by directly defining a prognostic signature. The statistical analyses will assess the performance of the models combining genomic instability, DNA Damage Response mutations and/or molecular subtyping to predict outcome after neoadjuvant chemotherapy and compare them with the models based on WSI deep learning approaches. These results will help to design new therapeutic strategies.
Interventions
COMBINATION_PRODUCTneoadjuvant chemotherapy with cisplatine
Blood from patient having muscle invasive bladder cancer who benefit from neoadjuvant chemotherapy with cisplatine
Sponsors
Institut Curie
University Hospital, Rouen
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
\- Inclusion Criteria of Patients enrolled in VESPER study: Blood and tumors from patient having muscle invasive bladder cancer who benefit from neoadjuvant chemotherapy with cisplatine included in VESPER cohort and : having signed an informed consent form for the participation to the collection or dead/lost to follow-up without prior opposition expressed against research program, genetic analysis will only be carried out for patients who have signed the genetic consent form. \- Inclusion Criteria of Patients from St Louis cohort not enrolled in VESPER study: Blood and tumors from patient having muscle invasive bladder cancer who benefit from neoadjuvant chemotherapy with cisplatine included in Saint-Louis cohort and : being informed for the participation to the collection and not having expressed opposition against research program or dead/lost to follow-up without prior opposition expressed against research program, \- Inclusion Criteria of Patients from St Louis cohort not enrolled in COBLaNCE study: Blood and tumors from patient having muscle invasive bladder cancer who benefit from neoadjuvant chemotherapy with cisplatine included in COBLaNCE cohort and : being informed for the participation to the collection and not having expressed opposition against research program or dead/lost to follow-up without prior opposition expressed against research program,
Exclusion criteria
* Patients not fulfilling eligibility criteria
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Progression-free survival | 3 years | The time from enrollment to progression or death |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Overall survival | 5 years | The time from enrollment to death |
Countries
France
Outcome results
None listed