Pregnancy Complications
Conditions
Keywords
Preeclampsia, PPROM, preterm birth, Fetal death, Low birthweight
Brief summary
In normal pregnancies, blood histamine concentration remains within normal values; however, the serum histaminolytic activity is increased. Histamine is mainly produced by mast cells, platelets, basophils, neurons and enterochromatin cells, where it is stored and released when required. Reduced DAO activities have been found in high-risk pregnancies. Lower DAO activity has been associated to miscarriage, fetal demise, preeclampsia, low birthweight and preterm birth. The placenta is very rich in DAO activity and is the principal source of increased histaminolytic activity in normal pregnancies. First-trimester screening for preeclampsia (PE) is a routine screening which is performed in the first trimester. Women with a positive result are at a greater risk of PE but also other pregnancy complications such as stillbirth, preterm birth and low birthweight. These cases are prescribed aspirin until term. This intervention has demonstrated to significantly reduce the risk of PE and other complications. Unfortunately, aspirin does not avoid all cases with adverse outcomes, indicating that additional mechanisms may be involved in the development of such pregnancy complications, such as DAO decreased activity.
Interventions
None listed
Sponsors
DR Healthcare
Hospital Universitari Vall d'Hebron Research Institute
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Single pregnancy * Maternal age ≥18 * High risk (≥1/50) in the first-trimester screening for preeclampsia * Gestational age between 11+0 and 16+6 weeks of gestation
Exclusion criteria
* Fetal malformation * Fetal death * Maternal digestive inflammatory disease or intolerance * Refusal to give informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Diamine oxidase activity first trimester | 11-16+6 weeks of gestation | diamine oxidase enzime activity in maternal blood |
| Diamine oxidase gene polymorphisms | 11-16+6 weeks of gestation | variants of the AOC1 gene: rs10156191, rs1049742, rs1049793 and rs2052129 |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Blood histamine levels at delivery | At the time of delivery | histamine levels in maternal blood |
| Blood Diamine oxidase activity at delivery | At the time of delivery | diamine oxidase enzime activity in maternal blood |
| Urine histamine levels at delivery | At the time of delivery | histamine levels in 24-hour maternal urine |
| Placental histamine levels at delivery | At the time of delivery | histamine levels in placenta |
| Placental Diamine oxidase activity at delivery | At the time of delivery | diamine oxidase enzime activity in placenta |
Countries
Spain
Outcome results
None listed