Microbial Colonization, Microbial Disease, Pneumonia
Conditions
Brief summary
The purpose of the protocol is to study the pulmonary microbiome in patients who develop pneumonia.
Detailed description
The purpose of the protocol is to study the pulmonary microbiome in patients who develop pneumonia with severe acute respiratory failure, requiring admission to the Intensive Care Unit, and to compare it with that of patients diagnosed with pneumonia but who do not develop severe acute respiratory failure and subsequent admission to the intensive care unit.
Interventions
PROCEDUREBronchoscopy
Through a bronchoscopic examination performed at the time of admission for diagnostic purposes, an alveolar bronchial lavage was performed for the analysis of the lung microbiome.
Sponsors
University Magna Graecia
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with acute respiratory failure * Patients with suspect of pneumonia
Exclusion criteria
* pregnancy * age \< 18 years
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Assessment of Lung Microbiome in Acute Respiratory Failure | The study will be conducted over a period of two months, during which data collection, bronchoscopic examinations, and analysis of the lung microbiome will be carried out. | This study aims to investigate the composition and diversity of the lung microbiome in patients experiencing acute respiratory failure. Specifically, the research evaluates differences in the lung microbiome between ICU-admitted patients, non-hospitalized individuals, and a control group without known lung pathologies. Through bronchoscopic examinations and alveolar bronchial lavage, the study seeks to elucidate potential associations between lung microbiota and the development of acute respiratory failure. For the analysis of the lung microbiome, the MiSeq Reagent Nano v2 kit is utilized. This kit enables high-throughput sequencing of microbial DNA extracted from samples obtained via bronchoscopic examinations and alveolar bronchial lavage. The MiSeq platform provides accurate and comprehensive data on the microbial composition and diversity present in the lung microbiome of study participants. |
Countries
Italy
Contacts
Primary ContactAndrea Bruni
Outcome results
None listed