Relationship Between Vitamins and Radiologic Stage in Knee Osteoarthritis

Investigation of the Relationship Between Vitamin B12, Folic Acid and 25 OH D Levels and Radiologic Stage in Knee Osteoarthritis

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT06267053

Enrollment

104

Registered

2024-02-20

Start date

2023-12-01

Completion date

2024-01-20

Last updated

2024-02-20

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Knee Osteoarthritis

Keywords

Vitamin B12, Folate, Vitamin D, Gonarthrosis

Brief summary

The aim of this study is to investigate the relationship between folic acid, vitamin B12 and 25-OH vitamin D levels and radiographic staging in patients diagnosed with primary gonarthrosis.

Detailed description

While in the past osteoarthritis (OA) was believed to be a degenerative disease of cartilage alone, recently, many factors such as trauma, mechanical strain, inflammation, biochemical reactions, and metabolic irregularities have been shown to be involved in its etiology. Until now there have been many studies done to investigate the relationship between serum 25-OH Vitamin D (25(OH)D) levels and knee OA however the relationship between folic acid, vitamin B12 and knee OA has been rarely studied. Therefore, the aim of this study is to investigate the relationship between folic acid, vitamin B12 and 25(OH)D levels and radiographic staging in patients diagnosed with primary gonarthrosis. The files of patients who presented to Sadi Konuk Training and Research Hospital's outpatient clinic of Physical Medicine and Rehabilitation with knee pain between June and December 2023 and were diagnosed with gonarthrosis according to the American College of Rheumatology (ACR) classification criteria were retrospectively reviewed. Either one way ANOVA or Kruskal Wallis H test was used to compare means determined based on the result of the Shapiro Wilk test. Bonferroni PostHoc was used to compare between groups. The statistical analysis was performed with Statistical Package for the Social Sciences (SPSS) version 21 (New York, USA) with significance set at p \< 0.05.

Interventions

DIAGNOSTIC_TESTDirect digital radiography

All the participants had anterior posterior and lateral knee xray taken within 3 months of diagnosis

DIAGNOSTIC_TEST25-OH Vitamin D

All the participants had serum 25-OH Vitamin D levels measured from venous blood within 30 days of diagnosis

DIAGNOSTIC_TESTVitamin B12

All the participants had serum Vitamin B12 levels measured from venous blood within 30 days of diagnosis

DIAGNOSTIC_TESTFolic acid

All the participants had serum folic acid levels measured from venous blood within 30 days of diagnosis

Study design

Observational model

CASE_ONLY

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

50 Years to 80 Years

Healthy volunteers

Inclusion criteria

* Adults (Age: 50-80) with primary gonarthrosis * Anterior-posterior and lateral knee direct digital radiography within 3 months of diagnosis * Vitamin B12, folic acid and 25-OH Vitamin D within 1 months of diagnosis

Exclusion criteria

* History of inflammatory or rheumatologic disease * History of lower extremity surgery * Pregnancy * Active malignancy * Severe mental disability

Design outcomes

Primary

Measure	Time frame	Description
25-OH Vitamin D (25(OH)D) and Kellgren Lawrence Grade	Baseline	One way ANOVA was used to determine the relationship between 25(OH)D and radiographical grade.
Vitamin B12 and Kellgren Lawrence Grade	Baseline	Kruskal Wallis H Test was used to determine the relationship between Vitamin B12 and radiographical grade.
Folic acid and Kellgren Lawrence Grade	Baseline	Kruskal Wallis H Test was used to determine the relationship between Folic acid and radiographical grade.

Countries

Turkey (Türkiye)

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026