Peri-implantitis, Periodontitis
Conditions
Keywords
Peri-implantitis, Periodontitis
Brief summary
1. To investigate the transcriptomic profile of periodontitis and peri-implantitis. Patients with defined periodontitis, at two non-adjacent teeth and peri-implantitis at ≥ one implant in function of ≥ one year, will be included to investigate the gene expression profile in tissue affected by periodontitis and peri-implantitis. Subjects will undergo a full-mouth examination performed by a calibrated examiner, including assessment of caries, bone abnormalities and infections according to intraoral x-ray, plaque index and pocket probing depth, suppuration, bleeding on probing, alveolar bone loss, and the number of teeth. During surgical intervention, tissue biopsies (two specimens per site) will be collected by a periodontist from site with ongoing periodontitis and site from ongoing peri-implantitis. 2. To study the microbiome and biomarker profile associated with periodontitis and peri- implantitis. Patients with defined periodontitis, at two non-adjacent teeth and peri-implantitis at ≥ one implant in function of ≥ one year, will be included in this study to determine the bacteria composition, cytokine profile and inflammatory biomarkers profile. Subjects will undergo a full-mouth examination performed by a calibrated examiner, including assessment of caries, bone abnormalities and infections according to intraoral x-ray, plaque index and pocket probing depth, suppuration, bleeding on probing, alveolar bone loss, and number of teeth. Peri-implant crevicular fluid (PICF), gingival crevicular fluid (GCF), saliva and submucosal/subgingival plaque will be collected.The presence and composition of periodontal and peri-implant plaque samples are investigated.
Interventions
OTHERPeriodontitis and Peri-implantitis
Defined periodontitis at ≤ 2 non-adjacent teeth AND Defined peri-implantitis at ≤ 1 implant, in function of at least 1 year.
Sponsors
Karolinska Institutet
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
≥18 years of age Defined periodontitis at two non-adjacent teeth Defined peri-implantitis at ≥1 implant Signed informed consent
Exclusion criteria
Surgical intervention of periodontitis and/or peri-implantitis during last 6 months. Local or systemic use of antibiotics during last 6 months.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Protein levels in peri-implantitis and periodontitis | Through study completion, an average of 1 years | To measure protein levels in gingival crevicular fluid and in peri-implant crevicular fluid. |
| Microbiome composition in peri-implantitis and periodontitis using 16S-rRNA sequencing method. | Through study completion, an average of 1 years | The microbiome composition will be analyzed in samples of subgingival/submucosal plaque using 16S-rRNA sequencing method. |
| Gene expression profile in peri-implantitis and periodontitis. | Through study completion, an average of 1 years | Soft tissue specimens will be studied in regards of gene expression levels using RNA sequencing method (Ilumina). The mRNA expression of candidate genes will be studied at protein level using immunohistochemistry. |
Countries
Sweden
Contacts
Primary ContactCarl Titusson, DDS, MSc
Outcome results
None listed