Periodontitis
Conditions
Brief summary
Periodontitis is a chronic inflammatory disease of the tooth supporting structures induced by a dysbiosis in the oral and subgingival microenvironment of susceptible patients. The long-term swallowing of high doses of periodontal pathogenic microorganisms could induce a dysbiosis of the intestinal microbiota, favouring the establishment of an 'inflamed' microbiome in terms of composition and/or function. The present project is aimed at a better understanding of the etiopathogenetic correlation between periodontitis and intestinal dysbiosis, and aims to explore the hypothesis that periodontal treatment may influence the multi-omics profile on the oral-gut-systemic axis. 70 patients affected by stage III-IV periodontitis will be recruited, and treated by means of full-mouth scaling and root planing. Salivary, subgingival plaque, plasma and stool samples, together with a complete periodontal charting and a food diary will be collected and compared at baseline and after treatment. Age, gender and BMI-matched healthy individuals will be recruited as controls.
Interventions
PROCEDUREActive periodontal treatment
Subgingival instrumentation with ultrasonic devices and curettes of all periodontal pockets; periodontal surgery if needed (residual probing pocket depths ≥ 6 mm).
Sponsors
University of Turin, Italy
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* periodontitis Stage III or IV * BMI between 20 and 29 kg/m2 * free diet * presence of at least 20 teeth
Exclusion criteria
* systemic diseases (including diabetes, thyroid, liver, or kidney diseases) * dietary allergies * use of antibiotics or probiotics during the previous 90 days * pregnancy or breast feeding
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes in gut microbial profile measured in stool samples | Baseline and 90 days | Taxonomic variation of gut bacteria concentration after treatment assessed through shotgun metagenomics |
| Changes in oral microbial profile measured in saliva samples | Baseline and 90 days | Taxonomic variation of salivary bacteria concentration after treatment assessed through shotgun metagenomics |
| Changes in oral microbial profile measured in subgingival plaque samples | Baseline and 90 days | Taxonomic variation of subgingival bacteria concentration after treatment assessed through shotgun metagenomics |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in oral-gut metabolome measured in stool samples | Baseline and 90 days | Untargeted metabolic variation of oral-gut environment after treatment |
| Changes in oral-gut miRNAome measured in stool samples | Baseline and 90 days | miRNA variation of oral-gut environment after treatment |
| Metabolic plasma changes | Baseline and 90 days | Concentration of IL-1β, IL-6, IL-10, TNF-α, INF-γ, glucose, and cortisol released in plasma |
Countries
Italy
Contacts
Primary ContactMario Aimetti, PA
Outcome results
None listed