Skeletal Muscle Damage
Conditions
Keywords
Recovery, Soccer, Soccer training, performance, inflammation
Brief summary
This study aims at investigating the recovery kinetics of skeletal muscle damage, neuromuscular fatigue and performance following a single soccer trainning session in middle aged males. The participants will perform a soccer training session \[A single training session including 60 minutes of warm up, soccer technical exercises and small-sided game\] and a cotrol trial (No intervention included, only daily measurements) in randomized, repeated measures, crossover design. Assesesments related to skeletal muscle damage, performance and neuromuscular fatigue will be executed before the training session and daily for four consecutive days after training.
Detailed description
Eleven male middle-aged participants will be included in this study. Participants will be initially informed about the main goals of study as well as the associated risks and benefits and then will provide their signed consent form. At baseline, they will undergo assessment of their anthropometrics (body mass, height, BMI, WHR), body compotition (using DXA), physical contition level (peak oxygen consumption, VO2peak), haemodynamic profile (diastolic and diastolic blood pressure) and daily dietary intake.Thereafter, they will participate in two experimental trials (Control trial and Soccer trial) in a randomized, crossover, repeated measure design: 1) ST (Soccer Trial): Participants in this trial will perform a soccer training session (60 min) consisted of warm-up, technical exercises and small-sided game, CT (Control Trial): Participants in this trial will only execute the evaluations daily in the dependent variables (they will not receive any intervention). Before each trial, the participants will provide a resting blood sample (for the determination of white blood cells, granulocyte cells, monocyte cells, lymphocyte cells, Hemeatocrit, Hemoglobin, red blood cells and platelets, total antioxidant capacity, glutathione and creatine kinase activity) and undergo assessments of their delay onset muscle soreness (DOMS), maximal voluntary isometric contraction (MVIC) of knee extensors and flexors (by isokinetic dynamometer), height and power of countermovement jump (CMJ-using force platform), 10m and 30m sprint time (will be evaluated using light cells). A 7-day wash-out period will apllied between ST and CT. The blood sample will be collected before and 24, 48 and 72 hours post-taining. Also, the evaluation of DOMS, MVIC, sprinting performance, power and jump height (by CMJ) will be evaluated before and 24, 48, 72 and 96 hours post training.
Interventions
OTHERST
Participants will perform a soccer training (ST) session for 60 minutes. The training will include the warm-up, soccer technical exercises and a small-side game
Sponsors
University of Thessaly
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
40 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Free of musculoskeletal injuries * No use of ergogenic supplements or medication * Free of chronic diseases * age of 40-60 years
Exclusion criteria
* Musculoskeletal injury * Use of alcohol, caffeine and any type of ergogenic supplements or medication during the course of the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in delayed onset of muscle soreness | At baseline, at 24, 48, 72 and 96 hours after soccer training | Muscle soreness will be assessed by palpation of muscle belly and the distal region |
| Change in Monocyte cell count | At baseline, at 24, 48 and 72 hours after soccer training | Monocytes cell count will be measured using an automatic blood analyzer |
| Change in Lymphocyte cell count | At baseline, at 24, 48 and 72 hours after soccer training | Lymphocyte cell count will be measured using an automatic blood analyzer |
| Change in percentage of Hematocrit level | At baseline, at 24, 48 and 72 hours after soccer training | The percentage of Hematocrit level will be measured using an automatic blood analyzer |
| Change Red blood cells count | At baseline, at 24, 48 and 72 hours after soccer training | Red blood cells count will be measured using an automatic blood analyzer |
| Change in Hemoglobin level | At baseline, at 24, 48 and 72 hours after soccer training | Hemoglobin level will be measured using an automatic blood analyzer |
| Change in Creatine kinase activity | At baseline, at 24, 48 and 72 hours after soccer training | Creatine kinase activity will be measured in plasma |
| Change in Total antioxidant capacity | At baseline, at 24, 48 and 72 hours after soccer training | Total antioxidant capacity will be measured in plasma |
| Change in Glutathione concentration in blood | At baseline, at 24, 48 and 72 hours after soccer training | Glutathione concentration will be measured in red blood cells |
| Change in countermovement jump height | At baseline, at 24, 48, 72 and 96 hours after soccer training | countermovement jump height will be measured using a force platform |
| Change in Peak power during coutermovement jump test | At baseline, at 24, 48, 72 and 96 hours after soccer training | The peak power will be measured using a force platform |
| Change in isometric peak torque of knee extensors and flexors | At baseline, at 24, 48, 72 and 96 hours after soccer training | Isometric peak torque will be assessed on an isokinetic dynamometer |
| Change in sprint time of 10 m | At baseline, at 24, 48, 72 and 96 hours after soccer training | 10m sprint time will be assessed using light cells |
| Change in sprint time of 30 m | At baseline, at 24, 48, 72 and 96 hours after soccer training | 30m sprint time will be assessed using light cells |
| Change in White blood cell count | At baseline, at 24, 48 and 72 hours after soccer training | White blood cell count will be measured using an automatic blood analyzer |
| Change in Granulocyte cell count | At baseline, at 24, 48 and 72 hours after soccer training | Change in White blood cell count will be measured using an automatic blood analyzer |
| Change in Platelets cell count | At baseline, at 24, 48 and 72 hours after soccer training | Platelets cell count will be measured using an automatic blood analyzer |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Peak Maximal oxygen consumption (Peak VO2) | At baseline | Peak VO2 will be estimated by open circuit spirometry via breath by breath method |
| Body Mass | At baseline | Body Mass will be measured on a beam balance with stadiometer |
| Body Height | At baseline | Body Height will be measured on a beam balance with stadiometer |
| Body Fat | At baseline | Body Fat will be measured by using Dual-emission X-ray absorptiometry |
| Lean body mass | At baseline | Lean body mass will be measured by using Dual-emission X-ray absorptiometry |
| Bone mass density | At baseline | Bone mass density will be measured by using Dual-emission X-ray absorptiometry |
| Bone mass content | At baseline | Bone mass content will be measured by using Dual-emission X-ray absorptiometry |
| Field activity during the soccer training | During the 60 minutes soccer training (ST) session (72 hours after baseline measurements) | The field activity will be continuously monitored during the soccer training using global positioning system (GPS) |
| Heart rate during the soccer training | During the 60 minutes soccer training (ST) session (72 hours after baseline measurements) | Heart rate will be continuously monitored during the soccer training using heart rate monitors |
| Systolic Blood Pressure | At baseline | Systolic Blood Pressure will be examined using sphygmomanometer |
| Dystolic Blood Pressure | At baseline | Dystolic Blood Pressure will be examined using sphygmomanometer |
| Rest Heart Rate | At baseline | The rest heart rate will be estimated using a heart rate monitor |
| Dietary intake | At baseline | Dietary intake will be assesed over a 7-day period using diet recalls |
Countries
Greece
Outcome results
None listed