Anorexia Nervosa
Conditions
Brief summary
Experimental and genetic data, as well as brain imaging, support a role of the hypothalamic Arcuate nucleus neurons and their communication with surrounding microglia in anorectic conditions, but it has until recently not been possible to explore these cells at a molecular level in anorexia nervosa (AN) patients. This attributed to the obvious lack of valid tissue and non-invasive imaging techniques of high enough resolution. Stem cell models have evolved as a useful tool for the exploration of other neuropsychiatric disorders with a comparably high genetic contribution as AN. The investigators will here use patient-derived induced pluripotent stem cells (iPSCs) to profile Arc neurons and peripheral blood mononuclear cell (PBMC) derived microglia in AN, thus defining molecules to explore as drug targets.
Interventions
OTHERobservational
Profiling the function of iPSC derived Arcuate neurons and PBMC derived microglia
Sponsors
Karolinska Institutet
The Swedish Research Council
Study design
Observational model
CASE_CONTROL
Time perspective
OTHER
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Inclusion criteria
anorexia nervosa: * female * 18-45 years of age * AN restrictive diagnosis as main diagnosis * more than 5 years duration
Exclusion criteria
anorexia nervosa: \- Inclusion Criteria healthy control: * female * 18-45 years of age * BMI 18,5 - 25
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Microglia function and transcriptomic profiling | 2025 | phagocytic and immune activation capacity and whole transcriptomic profiling, anorexia nervosa vs healthy controls |
| Arcuate neuron function and transcriptomic profiling | 2026 | Electrical activity, transmitter release and whole transcriptomic profiling of iPSC derived Arcuate neurons, anorexia nervosa vs healthy controls |
Countries
Sweden
Outcome results
None listed