Periodontal Diseases
Conditions
Keywords
Periodontitis, Agg. actinomycetemcomitans, JP2 genotype, PCR, local delivery drug, probiotics
Brief summary
the research highlight the effectiveness of probiotics as adjuncts to SRP in the treatment of periodontitis stage III grade C molar-incisor pattern
Detailed description
Background data : Periodontitis is a common disease that represents a public health problem. Aggregatibacter actinomycetemcomitans (A. a ) especially the JP2 genotype plays a major role in the pathogenesis of periodontitis. The aim of this study is to evaluate the effect of topically applied probiotics on the management of periodontitis stage III grade C molar-incisor pattern. Methods: Sixty-five patients having periodontitis stage III grade C molar-incisor pattern. Screening for A. actinomycetemcomians (A.a) and the JP2 genotype was done using conventional polymerase chain reaction (PCR). Patients positive for (A. a) were randomly divided into two groups. Group I: received conventional periodontal treatment combined with topically applied probiotic, while Group II: received conventional periodontal treatment only. Both groups were evaluated microbiologically and clinically. Quantitative assessment of A. a levels was done by real time PCR (qPCR) at baseline as well as one week, 4 weeks, and 12 weeks after treatment. Clinical parameters were assessed before treatment as well as 3,6 and 9 months after treatment.
Interventions
intra-pocket formula (local delivery drug)
DRUGplacebo formula
intra-pocket formula (local delivery drug)
Sponsors
Al-Azhar University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
SUPPORTIVE_CARE
Masking
NONE
Intervention model description
Sixty-five patients having periodontitis stage III grade C molar-incisor pattern. Screening for A. actinomycetemcomians (A.a) and the JP2 genotype was done using conventional polymerase chain reaction (PCR). Patients positive for (A. a) were randomly divided into two groups. Group I: received conventional periodontal treatment combined with topically applied probiotic, while Group II: received conventional periodontal treatment only. Both groups were evaluated microbiologically and clinically.
Eligibility
Sex/Gender
ALL
Age
10 Years to 28 Years
Healthy volunteers
Yes
Inclusion criteria
* patients have periodontitis stage III grade C molar-incisor pattern .
Exclusion criteria
* Pregnant or nursing women, * smokers, * patients received periodontal therapy, * systemic antimicrobials or anti-inflammatory drugs in the last six * patients with unacceptable oral hygiene
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| quantitative assessment of A. actinomycetemcomians levels by picogram | at baseline (before treatment) used by conventional PCR | Microbiological evaluation by picogram |
| Assessment of clinical periodontal parameter : Probing pocket Depth (PPD) by mm | at base line , 1 , 3 , 6 months | Clinical evaluation mm |
| Assessment of clinical periodontal parameter : Clinical Attachment Level (CAL) by mm | at base line , 1 , 3 , 6 months | Clinical evaluation by mm |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| quantitative assessment of A. actinomycetemcomians levels by picogram | baseline ,1 ,3 and 6 months | microbiological by picogram |
Countries
Egypt
Outcome results
None listed