Overweight and Obesity, Postprandial Hyperglycemia, Type2diabetes
Conditions
Brief summary
The overall objective of this research is to determine the digestive faith and health impact of protein and fiber rich foods in different food matrices in type 2 diabetes risk population. This is achieved by controlled postprandial intervention where plasma amino acids, lipids, glucose metabolism, bile acids and microbial metabolites are evaluated as well as fecal microbiota and metabolites.
Detailed description
Detailed research aim of this controlled, randomized crossover postprandial clinical study are: 1. To study the impact of complex plant- and animal-based foods on glucose metabolism, acute inflammation, and markers of microbial fermentation of fiber and proteins. 2. To investigate the difference in the gut mediated metabolism induced by naturally occurring fiber fraction bound plant protein or by plant protein with added fiber when compared to animal protein. 3. To conduct in vitro experiments using fecal samples from participants in the meal study to investigate the distinctions between different animal and plant proteins on both host and microbial metabolites, as well as the function of the gut barrier.
Interventions
DIETARY_SUPPLEMENTHigh fiber and protein meal
In the intervention participants are assigned to eat high protein and fiber meal that differ in protein source or structure.
DIETARY_SUPPLEMENTControl
The control meal is low fiber and low protein meal.
Sponsors
University of Eastern Finland
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
30 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
* 30-65 years of age * BMI \>25 kg/m2 and \<35 kg/m2 * Waist circumference with females \>85 cm and with males \>100 cm
Exclusion criteria
* Certain chronic diseases requiring regular medication (e.g., type 2 diabetes, chronic liver, kidney, thyroid, or gastrointestinal diseases) * Pregnancy or breastfeeding * Gluten-free or vegan diet * Use of antibiotics within the last 12 months * Gastrointestinal surgery within the last two years * High alcohol consumption
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Plasma amino acids | 0-4 hours | Plasma free amino acids were measured at 0, 30, 60, 90, 180, and 240 minutes after consuming the study meal. |
| Gut derived metabolites | 0-24 hours | Gut derived metabolites are measured with untargeted metabolomics from plasma at 12 hours after consuming the study meal. From fecal sample, the metabolomics are measured at 24 hours after consuming the study meal. Plasma short chain fatty acids are quantified at 12 hours after consuming the study meal. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma triglycerides | 0-4 hours | Plasma triglycerides are measured at 0, 30, 60, 90, 180, and 240 minutes after consuming the study meal. |
| Urine para-cresol | 24 hours | The amount of para-cresol is measured from urine sample at 24 hours after consuming the study meal. |
| Plasma glucose | 0-4 hours | Plasma glucose is measured at 0, 30, 60, 90, 180, and 240 minutes after consuming the study meal. |
| Plasma free fatty acids | 0-4 hours | Plasma free fatty acids are measured at 0, 30, 60, 90, 180, and 240 minutes after consuming the study meal. |
| Plasma insulin | 0-4 hours | Plasma insulin is measured at 0, 30, 60, 90, 180, and 240 minutes after consuming the study meal. |
| Inflammation marker | 0-4 hours | Plasma IL-22 is measured at 0, 60, 240 minutes after consuming the study meal. |
Countries
Finland
Contacts
Primary ContactMarjukka Kolehmainen, Professor
Backup ContactMoona Partanen, Msc
Outcome results
None listed