Socket Preservation
Conditions
Brief summary
Thirty patients will be selected from outpatient clinic of the Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Mansoura University seeking for extraction of non-restorable mandibular posterior teeth For the 1st group: the socket will be grafted with completely demineralized dentin graft after 3 days of extraction, followed by delayed implant placement after 4 months. For the 2nd group: the socket will be grafted with partially demineralized dentin graft in in the same day of extraction, followed by delayed implant placement after 4 months.
Interventions
PROCEDUREsocket preservation with completely demineralized dentin graft
socket preservation with completely demineralized dentin graft followed by delayed implant placement after 4 months
PROCEDUREsocket preservation with partially demineralized dentin graft
socket preservation with completely demineralized dentin graft followed by delayed implant placement after 4 months
Sponsors
Mansoura University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Patient medically free from systemic diseases. * Patient with good oral hygiene. * Age above 18 years. * Presence of non-restorable mandibular premolar tooth due to caries, root resorption, roots fracture, etc. * No acute infection is present. * No history of bruxism. * Patients prepared to comply with the follow-up and maintenance program.
Exclusion criteria
* Patients with systemic diseases that interfere with dental implant insertion. * Pregnancy. * Immunocompromised patients. * Existence of non-treated generalized progressive periodontitis.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Histomorphometric evaluation: | 4 monhs | After 4 months of socket preservation and just before implant placement, a trephine bur will be used to collect a core of bone from the preserved socket. Bone will be examined histologically to evaluate the quality and quantity of the formed bone. Briefly, the specimens were fixed with 4% paraformaldehyde in 0.1M PBS (pH 7.4) and decalcified in 0.5M EDTA (pH 7.8). After decalcification, each specimen will be processed and embedded in paraffin, and transverse serial sections of 5 μm thickness will be prepared. The sections will be stained with hematoxylin and eosin (H&E), Masson's Trichrome (MT) (Cat # HT15, Sigma Aldrich) and immunohistochemical staining with osteocalcin antibody polyclonal osteocalcin (OCN);diagnostic biosystems), histological examination will be carried out using an Olympus BX-51 optical microscope (Olympus Co., Tokyo, Japan). |
| Histomorphometric analysis | 4 months | Microscopic images of the surgical sites will be taken by randomly selecting four areas of the center portion of the defect at 100× magnification. Then, using 16 images of each group, the ratio of newly formed bone to the entire defect area in the slide images will be calculated in trichrome and osteocalcin-stained images using the Intel® Core I7® based computer using VideoTest Morphology® software (Russia) with a specific built-in routine for % area, measurement. |
| Bone density: | 12 months | Bone density will be evaluated using Cone Beam Computed Tomography (CBCT) that will be made immediately after socket preservation, at 4 months after preservation (just before implant placement), and after 12 months from implant loading. |
Countries
Egypt
Outcome results
None listed