Multiple Myeloma
Conditions
Keywords
Multiple Myeloma, Circulating plasma cells, Multiparameter flow cytometry, Prognosis, Disease monitoring
Brief summary
This study aims to investigate the prognostic value of circulating plasma cells (CPCs) in patients with multiple myeloma and explore whether CPCs detection might be used in place of bone marrow aspiration for disease monitoring.
Detailed description
Circulating plasma cells (CPCs) represents a phenotypic subset of bone marrow multiple myeloma (MM) cells, which would contribute to the progression and dissemination of the tumor. High-sensitivity techniques such as multiparameter flow cytometry provide a tool for better detection of CPCs; however, a clear threshold has not been identified. The primary objective of the current study is to identify an optimal threshold for CPCs quantified by 8-color flow cytometry (with antibodies to CD38, CD138, CD45, CD56, CD19, CD27 and cytoplasmic kappa and lambda immunoglobulin light chains) and determine the specific relationship between CPCs level and the prognosis of MM. In addition, the evaluation of CPCs at multiple time points will be performed to explore whether CPCs detection might be used in place of bone marrow aspiration for disease monitoring.
Interventions
We will draw 2-5 mL of peripheral blood and utilize multiparameter flow cytometry to measure the level of circulating plasma cells.
Sponsors
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
Patients must meet all of the following inclusion criteria. * Newly diagnosed multiple myeloma patients. * Patients without any previous anti-myeloma treatment. * Age: 18-80years old (adult). * No history of cancer. * Informed consent.
Exclusion criteria
Patients meeting any of the following
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 2-year progression-free survival (PFS) | 2 years | To evaluate if there is a correlation between high CPCs level and poor PFS. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Rates and depth of response | 2 years | To evaluate if there is a correlation between CPCs level and rates and depth of response (IMWG response criteria). |
| Overall survival (OS) | 2 years | To evaluate if there is a correlation between high CPCs level and poor OS. |
Countries
China
Contacts
Primary ContactChunyan Sun
Outcome results
None listed