Healthy
Conditions
Brief summary
The present study aims to quantify the insulin sensitivity and the skeletal muscle protein synthetic response to consuming a test drink containing all amino acids or all amino acids except for BCAAs.
Detailed description
Twenty-eight healthy, young volunteers will receive a stable isotope tracer infusion (7.5h) combined with repeated blood and muscle sampling, to measure muscle protein synthesis rate in the postabsorptive state following ingestion of a drink containing all amino acids (n=14) or all amino acids except for BCAAs (n=14).
Interventions
DIETARY_SUPPLEMENTIngestion of an amino acid supplement
Oral intake of a supplement containing amino acids
Sponsors
Wageningen University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Aged from 18-35 years * 18.5 \< Body mass index \< 30 kg·m2 * Recreationally active (performing non-competitive physical exercise at least one time per week for minimally 30 minutes)
Exclusion criteria
* Smoking * Diabetes (Type 1, Type 2, or genetic form of diabetes) * Any diagnosed cardiovascular (heart) disease or high blood pressure (≥140 mmHg systolic and/or ≥90 mmHg diastolic) * Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices) * Known allergy to lidocaine * Regular use of dietary protein and/or amino acid supplements (\>3 times per week) * Currently involved in a structured progressive resistance training programme (\>3 times per week) * A personal or family history of thrombosis (clots), epilepsy, seizures, or schizophrenia. * Any previous motor disorders or disorders in muscle and/or lipid metabolism * Presence of an ulcer in the stomach or gut and/or strong history of indigestion * Known severe kidney problems * Pregnant or breastfeeding * Unable to give consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Postprandial muscle protein synthesis | 4 hours | Muscle protein synthesis rate (FSR) prior to and following ingestion of an amino acid drink |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Forearm amino acid kinetics | Prior to and for 4 hours following amino acid intake | Plasma phenylalanine kinetics using a stable isotope tracer infusion |
| Forearm muscle glucose uptake | 7.5 hours | Insulin sensitivity, measured as forearm glucose uptake, prior to and following ingestion of an amino acid drink |
| Whole-body insulin sensitivity | Four hours following ingestion of an amino acid drink | Whole-body insulin sensitivity, calculated using a modified Matsuda index |
Countries
Netherlands
Outcome results
None listed