Glycemic Control, Insulin Sensitivity, Sex Differences
Conditions
Brief summary
The purpose of the proposed study is to compare the acute effects of different types of exercise modalities on glucose handling in young, healthy males and females. The exercise modalities that will be compared include: a high intensity interval exercise (HIIE) protocol, a moderate intensity continuous exercise (MICE) protocol and a low-load, high-repetition (LL-HR) resistance exercise protocol.
Detailed description
The purpose of this study is to compare the effects of different exercise modalities (High intensity interval exercise, Moderate intensity continuous exercise, and Low-load-high repetition resistance exercise) on blood sugar control in young healthy, males and females. To test this theory, a sugar challenge (oral glucose tolerance test - OGTT) will be performed during the pre-testing visit to determine the baseline blood sugar response. An OGTT will also be performed 2 hours after each single exercise session to determine how each type of exercise affects the blood sugar response. Investigators will take multiple blood samples throughout the trial to allow us to measure blood sugar, insulin and concentrations and oxidative stress. Muscle biopsies will be taken before and after each exercise bout so that investigators can examine the underlying mechanisms that contribute to the effectiveness of the different types of exercise on blood sugar control. Investigators will also use an ultrasound to measure factors related to of cardiovascular health such as artery stress and vascular conductance during the exercise bouts. Direct benefits of this study include characterizing sex differences of blood sugar handling after single exercise sessions. This study could have direct benefits on deterring the effectiveness of different forms of exercise on blood sugar levels.
Interventions
OTHERControl Oral Glucose Tolerance Test
control oral glucose tolerance test
OTHERMixed Exercise
exercise performed in randomized order
Sponsors
University of Waterloo
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* In order to participate in this study, participants must be a healthy man or woman between the ages of 18 and 30 years.
Exclusion criteria
* Presence of chronic health condition(s) \[i.e. metabolic (i.e. Type 1 or type 2 diabetes), cardiovascular (i.e hypertension), respiratory (i.e. chronic pulmonary obstructive disorder) or digestive (i.e. ulcerative colitis) disorders. * Are pregnant, or suspect that they may be pregnant, or nursing * Inability to complete the single exercise sessions. * Regularly participate in cardiovascular (\>3 sessions/week) or resistance training (\>2 sessions/week) exercise. * Have an allergy to local anesthetic (or family history of allergy) * Have undergone a barium swallow or an infusion of a contrast agent in the past 3 weeks * Are taking prescription anti-coagulant or anti-platelet medications (e.g., warfarin, heparin, clopidogrel) * Inability to exercise as suggested by the get active questionnaire (GAQ) * BMI \> 27 kg/m2
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood insulin change | 120mins | To assess the effect of sex and exercise on basal and post-exercise blood insulin |
| Blood glucose change | 120mins | To assess the effect of sex and exercise on basal and post-exercise blood glucose. |
| Blood c-peptide change | 120mins | To assess the effect of sex and exercise on basal and post-exercise blood c-peptide. |
| Insulin sensitivity change | 120mins | Assess the changes in insulin sensitivity pre to post exercise |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| glucose transporter 4 change (GLUT4) | 35mins | examine protein content changes from pre to post exercise |
| TBC1 domain family member 4 change (TBC1D4) | 35mins | examine protein content changes from pre to post exercise |
| progesterone concentration | 30mins | to assess the differences in progesterone concentration between males and females |
| estrogen concentration | 30mins | to assess the differences in estrogen concentration between males and females |
| TBC1 domain family member 1 change (TBC1D1) | 35mins | examine protein content changes from pre to post exercise |
| protein kinase B change | 35mins | examine protein content changes from pre to post exercise |
| 5' adenosine monophosphate-activated protein kinase change | 35mins | examine protein content changes from pre to post exercise |
Countries
Canada
Outcome results
None listed