Immune Heterogeneity Before and After Cardiopulmonary Bypass in Children

A Study of Immune Heterogeneity Before and After Cardiopulmonary Bypass in Children Undergoing Cardiac Surgery Based on ScRNA-seq

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT06214182

Enrollment

Registered

2024-01-19

Start date

2024-01-01

Completion date

2025-01-31

Last updated

2025-03-11

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Ventricular Septal Defect

Brief summary

The goal of this observational study is to learn about functional heterogeneity of immune cells before and after cardiopulmonary bypass (CPB) in children with congenital heart disease (CHD). The main question\[s\] it aims to answer are: * Does CPB cause immune paralysis after CHD surgery? * How does the functional heterogeneity of immune cells change before and after CPB in children undergoing CHD surgery? Participants will receive 3ml of peripheral blood before CPB, 2 hours after CPB, and 3 days after CPB, and the peripheral blood samples will be sequenced by single cell to explore the immune heterogeneity before and after CPB.

Detailed description

CPB-induced immune paralysis is the main cause of postoperative infection in CHD, which seriously affects the prognosis of children. The underlying mechanism of CPB-induced immune paralysis has not been fully determined. This study intends to use scRNA-seq to describe gene transcription profiles of immune cells in peripheral blood of children undergoing CHD surgery before and after CPB, and explore the differences within and between different immune cell populations including T cells and the changes of immune cells in different sequences. To fill the gap in the study of immune mechanism in CPB-induced immune paralysis microenvironment at single-cell resolution.

Interventions

None listed

Study design

Observational model

CASE_ONLY

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

31 Days to 1 Years

Healthy volunteers

Inclusion criteria

1. Birth weight range: 2500-6000g; 2. Operation duration \>90min;

Exclusion criteria

1. preterm infants or preoperative infections; 2. systemic immune diseases and serious complications: such as rheumatoid arthritis, systemic lupus erythematosus, severe liver and kidney function damage, leukemia, etc.; 3. Patients who have received an organ transplant; 4. Patients who have received immunomodulatory drugs: patients who have received immunomodulatory drugs before surgery; 5. Parents refused to participate or were unable to provide sufficient samples: Parents or legal guardians refused to participate in the study.

Design outcomes

Primary

Measure	Time frame	Description
Expression of genes	From enrollment to the end of CPB at 3 days	The gene expression in single cells of peripheral blood samples was detected

Countries

China

Outcome results