Acute Respiratory Failure, Critical Illness
Conditions
Keywords
exhaled breath condensate, proteomics, genomics, mechanical ventilation, acute respiratory failure, lung injury, intensive care
Brief summary
Mechanically ventilated intensive care patients will be sampled for a small amount of exhaled breath condensate from the ventilator circuit and for venous blood. Proteomic analysis of the exhaled breath condensate will be performed using mass spectrometry and in the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied. Resulting profiles will be correlated with routinely monitored parameters in order to identify patterns corresponding to various pathologies in order to enable their early detection.
Detailed description
Exhaled breath condensate (EBC) is a product of gas exchange in the lungs. It contains plethora of substances of possible diagnostic value and its composition is likely to change rapidly in response to the development of pathological conditions, especially in the lungs. Mechanically ventilated intensive care patients with various pathological conditions will be sampled for a small amount of EBC from the ventilator circuit and for venous blood. A sampling method not affecting relevant parameters of mechanical ventilation will be developed and standardised. EBC samples will be frozen and after processing, proteomic analyses will be performed using mass spectrometry. In the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied using molecular biological methods. Resulting profiles will be correlated with routinely monitored parameters (such as inflammation markers, microbiological findings and mechanical ventilation parameters) with the aim of finding patterns corresponding to the early stages of various pathological conditions focusing on lung tissue. Identification of patterns specific to initial stages of lung and other organ pathologies would enable their early treatment possibly shortening mechanical ventilation/ICU stay and reducing morbidity/mortality.
Interventions
DIAGNOSTIC_TESTProteomic analysis of exhaled breath condensate
Mechanically ventilated intensive care patients will be sampled for exhaled breath condensate from the ventilator circuit.
Sponsors
The Institute of Molecular and Translational Medicine, Czech Republic
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
Intensive care setting Mechanical ventilation Ventilator/ventilator circuit technically suitable for sampling of exhaled breath condensate
Exclusion criteria
Mechanical ventilation challenging to the extent that the insertion of the sampling kit into the ventilator circuit would substantially increase the risk for respiratory deterioration
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Comparison of proteomic patterns of the exhaled breath condensate in mechanically ventilated intensive care patients. | January 2024 - December 2029 | Proteomic patterns of the exhaled breath condensate will be studied in mechanically ventilated intensive care patients. The results will be analysed and compared with the aim to identify patterns specific for the pathological conditions. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - DNA | January 2024 - December 2029 | Venous blood sample will be analysed employing targeted analyses of DNA. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate. |
| Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - RNA | January 2024 - December 2029 | Venous blood sample will be analysed employing targeted analyses of RNA. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate. |
| Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - proteins | January 2024 - December 2029 | Venous blood sample will be analysed employing targeted analyses of proteins. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate. |
| Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - metabolites | January 2024 - December 2029 | Venous blood sample will be analysed employing targeted analyses of metabolites. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate. |
Countries
Czechia
Contacts
CONTACTEmil Berta, MD PhD
CONTACTKarel Axmann, MD
PRINCIPAL_INVESTIGATOREmil Berta, MD PhD
IMTM, Palacky University in Olomouc, Faculty of Medicine and Dentistry
Outcome results
None listed