Skeletal Muscle Physiology
Conditions
Brief summary
The purpose of this project is to investigate underlying mechanisms of testosterone- and resistance training-induced muscle hypertrophy and muscle memory in humans.
Interventions
DRUGTestosterone Undecanoate
Following testosterone/placebo injection, participants undergo supervised resistance training for 4 weeks. This period is followed by a washout period with no drug and either continued resistance training or detraining for 16 weeks. Thereafter, another 4-week period with supervised resistance training is commenced, with no drug.
Sponsors
Morten Hostrup, PhD
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women * BMI of \<26 and normal ECG and blood pressure
Exclusion criteria
* Smoking * Chronic disease, * Use of prescription medication * Pain due to current or previous musculoskeletal injury * Resistance training more than once per week in the 12 months leading up to the intervention * Current or previous use of prohibited anabolic substances
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fiber cross-sectional area | The change in fiber cross-sectional is assessed at baseline, 4, 20 and 24 weeks | Immunohistochemistry to determine fiber cross-sectional area |
| Myonuclei | The change in myonuclei is assessed at baseline, 4, 20 and 24 weeks | Immunohistochemistry to determine number of myonuclei |
| Skeletal muscle proteome | The change in the muscle proteome is assessed at baseline, 4, 20 and 24 weeks | Pathway enrichment analysis of the muscle proteome using mass spectrometry-based proteomics |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Lean mass | The change in lean mass is assessed at baseline, 4, 20 and 24 weeks | Lean mass assessed by dual X-ray absorptiometry (DXA) |
| Satellite cells | The change in satellite cells is assessed at baseline, 4, 20 and 24 weeks | Immunohistochemistry to determine number of satellite cells |
| Muscle strength | The change in 1-repetition maximum is assessed at baseline, 4, 20 and 24 weeks | 1-repetition maximum |
Countries
Denmark
Contacts
Primary ContactLukas Moesgaard
Outcome results
None listed