Proteomic Study of Plasma Exosomes in Patients With Diabetic Retinopathy

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT06188013

Enrollment

Registered

2024-01-03

Start date

2024-01-02

Completion date

2024-06-30

Last updated

2024-01-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Diabetic Retinopathy

Keywords

plasma, exosome, Diabetic Retinopathy

Brief summary

Objective: Explore the biomarkers related to proliferative diabetic retinopathy by analyzing the protein expression profile changes of plasma exosomes in patients with difference phases of diabetic retinopathy, and investigate potential molecular mechanisms and therapeutic targets. Methods: Enrich exosomes from plasma by column extraction method. NTA, WB and TEM were used to characterize the obtained exosomes, and exosome proteinomics was performed by chromatography⁃mass spectrometry. Corhorts: The study was divided into four groups: a healthy control group, a diabetic without diabetic retinopathy group, a non-proliferative diabetic retinopathy group, and a proliferative diabetic retinopathy group, with 6 cases in each group.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* Healthy volunteers without diabetes/ Senile cataract patients without diabetes； * Patients with diabetes who are diagnosed without DR, NPDR, and PDR by mydriatic fundus photography (or FFA)；

Exclusion criteria

* Patients with systemic diseases such as cancer, immune diseases, infectious diseases, and blood diseases； * Patients with cerebral infarction, myocardial infarction and other cardiovascular and cerebrovascular diseases

Design outcomes

Primary

Measure	Time frame	Description
differential proteins of exosomes	December,2023-June,2024	Exosomes were extracted and isolated from plasma, proteomics analysis of proteins in exosomes was performed to compare the number of types of changes in exosomal proteins of the four groups and to identify proteins that were up-regulated and/or down-regulated, and the relationship between the proteins and diabetic retinopathy was then analysed to look for possible mechanisms and therapeutic targets for diabetic retinopathy.

Contacts

Primary ContactHang Wu, Master

wuhang2317@163.com+86-13911993866

Backup ContactZhen Li, Doctor

zhenli_xwyy@163.com+86-13581646689

Outcome results