Diet, Healthy
Conditions
Keywords
Functional sympatholysis, Exercise
Brief summary
The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are: 1. What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal 2. Can dietary nitrate prevent the declines in exercise-mediated sympatholysis Participants will be asked to complete three study visits in a randomized order: 1. Low-fat meal 2. High-fat meal 3. High-fat meal plus dietary nitrate Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.
Interventions
DIETARY_SUPPLEMENTBeetroot drink
Drink containing 10g of beetroot powder in 250 mL cold tap water
DIETARY_SUPPLEMENTPlacebo
Mio flavouring in 250 mL cold tap water. Trace amounts of sugar and sodium were added to match the content in the beetroot supplement
DIETARY_SUPPLEMENTControl
Low-fat meal with cornflakes, greek yogurt, skim milk, and orange juice
Sponsors
University of Guelph
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* 18-30 years of age * Healthy, free of known cardiovascular, metabolic, or musculoskeletal disease
Exclusion criteria
* Diagnosed cardiovascular or metabolic disease, injury preventing handgrip exercise, prescription of chronic medications other than oral contraceptives. * Inability to abstain from drugs (except oral contraceptives), caffeine, alcohol, strenuous physical activity, mouthwash, and multivitamins prior to study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Forearm vascular conductance | Before intervention and 1, 2, 3 hours post-intervention | Duplex ultrasound of brachial artery |
| Forearm blood flow | Before intervention and 1, 2, 3 hours post-intervention | Duplex ultrasound of brachial artery |
| Systolic and Diastolic Blood Pressure | Before intervention and 1, 2, 3 hours post-intervention | finger plethysmography |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma levels of Norepinephrine | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date | ELISA kit |
| Plasma levels of Triglycerides | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date | Triglyceride assay kit |
| Plasma levels of Nitrate/Nitrite concentration | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date | ELISA kit |
| Blood Glucose concentrations | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood analyzed immediately after collection at each time point | Blood glucose monitor |
Countries
Canada
Outcome results
None listed