Bronchiolitis
Conditions
Keywords
Newborns, Dysbiosis, respiratory syncytial virus (RSV)
Brief summary
Acute bronchiolitis is a common disease in children under the age of two, caused mainly by the respiratory syncytial virus (RSV). Furthermore, given the same medical history, it is still very difficult to predict the course and severity of the infection at the onset of symptoms, Some studies have highlighted the importance of the microbiota (intestinal, oral or nasopharyngeal) and of the immune response to RSV in children, We will include 80 children under 2 years old with hospitalized bronchiolitis and non-hospitalized bronchiolitis. Oral, nasal and stool samples will be taken to study the various microbiota in search of dysbiosis. A capillary blood sample will be taken for immune studies.
Interventions
OTHERSampling
Single-time sampling of respiratory virus-infected children
Sponsors
Infectious Diseases Models for Innovative Therapies center
Assistance Publique - Hôpitaux de Paris
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
1 Days to 12 Months
Healthy volunteers
No
Inclusion criteria
* Infants \<12 months * With bronchiolitis during RSV epidemic season * No chronic illness * No bronchiolitis medical history * Signed consent from parents or legal guardians
Exclusion criteria
* Chronic respiratory illness * Medical history of bronchiolitis or newborn asthma * Treatment with immunosuppressants * Patient with no social security affiliation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Dysbiosis | Inclusion | comparison of the quantitative and qualitative composition of bacteria (alpha diversity, beta diversity, Shannon and Simpson index) in the digestive and nasopharyngeal microbiota |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Measurement of innate and adaptive responses by quantification of cytokines and chemokines in plasma | inclusion | Inflammatory/regulatory cytokines (IL1-\>17, IL-10, TGF-b, EGF, FGF-2, IFN-alpha, IFN-gamma, IFN-beta, MCRP-1 MCP-3, TNF-alpha...), cytokines of adaptative response (Th1, Th17, Th2 and/or Treg), markers of inflammatory response (interferon, TNFa, …), quantified by Luminex |
| Identifying the mRNA profile in blood samples and nasal swabs | inclusion | mRNA will be identified by using RNA-Seq (Illumina) |
| Sequencing viral strains for mutation | Inclusion | Typing of respiratory syncytial virus-A (RSV-A) and B (RSV-B) and sequencing of strains (Amplicons) |
| Comparison of respiratory syncytial virus (RSV) antibodies levels on newborn screening specimen and on capillary swab during infection | Birth, Inclusion | Levels of RSV antibodies will be measured and compared between newborn screening specimen and capillary swab during infection |
| Study the load of Streptococcus pneumoniae (Sp) in RSV infections. | Inclusion | Compare the serotypic profiles of pneumococcus (Sp) present during RSV infections |
Countries
France
Contacts
Primary ContactEtienne BIZOT, Doctor
Outcome results
None listed