Postprandial Amino Acid Concentrations in Healthy Adults
Conditions
Keywords
Postprandial, Healthy individuals, Amino acid, Bioavailability, Protein
Brief summary
This study assesses the amino acid concentrations in subject serum after intake of different types of proteins. The study is divided in two consecutive sub-studies. Subjects will be asked to ingest a specific protein blend each visit in a randomized order, after which blood will be drawn on 14 different time points. At least 48 hours will be kept between each visit, and the subjects receive a follow up call within 4-14 days after visit 4.
Interventions
DIETARY_SUPPLEMENTProtein products
In total 4 protein products will be tested containing a single or mixture of plant and dairy protein sources in a randomized order
Sponsors
Nutricia Research
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
1. Age ≥ 18 and ≤ 70 years 2. Body Mass Index (BMI) ≥ 18.5 and ≤ 27.0 kg/m2 3. Written informed consent 4. Willingness and ability to comply with the protocol 5. Judged by the Investigator to be in good health
Exclusion criteria
1. Any gastrointestinal (GI) disease or surgery that may interfere with GI function and/or protein metabolism, including but not limited to phenylketonuria, pancreatitis, short bowel syndrome, celiac disease, Crohn's disease, in the opinion of the Investigator 2. Known renal or hepatic diseases that may interfere with protein metabolism, including but not limited to acute hepatitis, chronic liver disease, nephritis, cystinuria, chronic kidney disease, in the opinion of the Investigator 3. Use of systemic antibiotics, anticonvulsants, prokinetics, antacids, medication influencing gastric acid production, systemic anticoagulants, systemic corticosteroids, laxatives, growth hormone, testosterone, immunosuppressants or insulin within the past 3 weeks prior to screening 4. Allergy to soy, pea and/or cow's milk protein 5. Adherence to a weight loss program 6. Current eating disorder, e.g. anorexia nervosa or bulimia 7. Known pregnancy and/or lactation 8. Current smoking or stopped smoking for \< 1 month prior to screening (except for incidental smoking of ≤ 3 cigarettes/cigars/pipes per week on average in the last month prior to screening) 9. Average alcohol use of \> 21 glasses per week for men or \> 14 glasses per week for women (on average during the last 6 months prior to screening) 10. Drug or medicine abuse in opinion of the investigator 11. Any known bleeding disorder 12. Known difficulties with placement of and/or blood drawings from a cannula 13. Active participation in any other study with investigational or marketed products concomitantly or within 4 weeks prior to screening 14. Major medical or major surgical event requiring hospitalization within the preceding 3 months and/or scheduled in the period of study participation 15. Investigator's uncertainty about the willingness or ability of the subject to comply with the protocol requirements 16. Employees of Nutricia Research and/or their family members or relatives
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Amino Acid concentration in blood | 240 minutes after dietary protein ingestion | Relative content of amino acids in dietary protein and relative amount of amino acids appearing in the blood for the 4 hour period after the ingestion of different products \[μmol\] |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Individual Amino Acid concentration in blood | 240 minutes after dietary protein ingestion | Postprandial serum concentrations of individual amino acids \[μmol/L\] |
Countries
Netherlands
Contacts
Primary ContactDanone Nutricia Research
Outcome results
None listed