Healthy Aging, Physical Inactivity
Conditions
Brief summary
The study will consist of three groups of subjects. Ten young male subjects (18-30 years) will lie supine for 21 days, 10 older male subjects (65+ years) will lie supine for 10 days, and a third group of 10 older male subjects (65+ years) will also lie supine for 10 days but will receive interventions (see below) to counteract the physical changes caused by supine lying. All three groups will be bedridden in the premises of Izola General Hospital. Subjects will be housed in 5-bed rooms at the hospital and will receive 24-hour medical care, proper hygiene, nutrition, passive exercise, Internet, a relaxing environment, and visits. After their stay, subjects return home but receive 21 days of care at local fitness centers. Rehabilitation consists of interval training, in which subjects perform high-intensity exercise for 4 times 5 minutes. A number of important health parameters are measured before, during, and after bed rest. Some of these measurements will be invasive (blood and muscle sampling), for which appropriate medical personnel, sterile instruments, and environment will be available. Measurements will include normal voluntary efforts (indoor cycling, various jumps, short stretches) and electrically evoked muscle contractions (electrical stimulation with single impulses). All tests are normal tests and come from normal lifestyles and routine medical examinations.
Interventions
DIETARY_SUPPLEMENTHigh protein diet
Test the ability of high protein diet (1.4 g protein/kg/day) with branched chain amino acid and cysteine supplementation to decrease bed rest induced muscle atrophy, glutathione depletion and insulin resistance in old subjects.
DIAGNOSTIC_TESTOGTT
Assessment of changes in insulin sensitivity (Oral Glucose Tolerance test and muscle insulin signalling: immunofluorescence and western blot of GLUT-4, IRS-1, MPP/TIMP, etc.) following bed rest in old and young subjects.
Sponsors
Azienda Sanitaria Universitaria Giuliano Isontina (ASU GI)
Izola General Hospital
University of Padova
University of Udine
University of Pavia
University of Trieste
Science and Research Centre Koper
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* age: for young arm (18-35), for old arm (65-75) * BMI: 22 in 32 kg/m2 * appendicular muscle mass \> 7,23 kg/m2
Exclusion criteria
* serious acute or chronic diseases: skeletal, cancer, cardiovascular, metabolic, neuromuscular and psychiatric; * cardiovascular disease detected on ECG examination; * allergies to the active substances used in the study; * problems with digestion of the active substances; * frequent diarrhea and vomiting; * arterial hypertension (\>140/90 mmHg); * psychological problems; * susceptibility to deep vein thrombosis (D-dimer \< 500 μg/L) * poor orthostatic tolerance (frequent dizziness when changing position, especially when rising from a supine position); * venous abnormalities that would cause difficulty in drawing blood; * a large blood volume (more than 300 ml) in the last 3 months prior to the start of the project; * problems with excessive consumption of alcoholic beverages; * active smoking; * Previous participation in any biomedical research in the last 3 months prior to the start of this study; * a communicable disease before the start of the project; * any ferromagnetic implant.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Insulin sensitivity | 10 days | Assessment in changes in insulin sensitivity will be made with the use of Oral Glucose Tolerance test (OGTT). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscle mass | 10 day | Muscle mass will be measured with bioimpedance and with DXA. |
| Muscle force | 10 days | Quadriceps femoris maximal voluntary contractions (MVC) in isometric conditions at knee angle set at 30 deg flexion and hip fixed at 90 deg flexion using a custom-made knee dynamometer fitted with a load cell (RS 206-0290). |
| Neuromuscular junction stability | 10 days | We will evaluate the presence of NMJ stability by analysing: AChR subunits expression |
Countries
Slovenia
Outcome results
None listed