Connective Tissue, Dietary Supplements, Exercise
Conditions
Brief summary
The purpose of this study is to test whether a natural product supplement can potentiate the increase in collagen synthesis following the ingestion of collagen protein. The investigators have developed a model of natural (GRAS certified) products that stimulate collagen synthesis, in vitro. The investigators will determine whether the natural product supplement can potentiate the collagen synthetic response to the ingestion of collagen protein. Basal and fed serum will be isolated and these samples will be used to treat human engineered ligaments.
Interventions
DIETARY_SUPPLEMENTNatural Product Supplement
Participants will have blood drawn before and one hour after consuming the natural product supplement. For 7-days the participants will perform load-bearing exercise and consume the supplement. On the final day, blood will be collected 4 hours after the final exercise bout.
DIETARY_SUPPLEMENTPlacebo control
Participants will have blood drawn before and one hour after consuming the placebo supplement. For 7-days the participants will perform load-bearing exercise and consume the supplement. On the final day, blood will be collected 4 hours after the final exercise bout.
Sponsors
University of California, Davis
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* Young healthy adults (18-30 y)
Exclusion criteria
* Pregnancy * Smoking * Receiving any medication that may interfere with the study outcomes
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Collagen protein synthesis | 7 days | Collagen protein synthesis will be indirectly measured by measuring pro-collagen type I N-terminal propeptide (PINP) in the serum before and 4 hours after the last exercise bout (at day 7 of the protocol). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Strength of ligament constructs | 60 minutes post-exercise | Serum from participants will be used to treat engineered ligaments in order to measure the effect of the supplementation on the strength of the ligaments. The strength will be measured using the Instron bio puls 68SC-1 tension and compression machine. |
| Collagen content of ligament constructs | 60 minutes post-exercise | Ligament constructs will be treated for 7 days with experimental feed medium containing serum obtained at baseline or post-prandial for determination of collagen content using a hydroxyproline assay. |
Countries
United States
Contacts
CONTACTKevin Paulussen, PhD
CONTACTKeith Baar, PhD
PRINCIPAL_INVESTIGATORKeith Baar, PhD
University of California, Davis
Outcome results
None listed