Fasting
Conditions
Keywords
Thermogenesis
Brief summary
Fasting reduces the energy consumption of the human body. The extent of this adaptation varies significantly between different individuals. The aim of this research project is to investigate how this adaptation of the metabolism is regulated by the body. For this purpose, we will first measure how the so-called basal metabolic rate of the body reacts to a short-term fasting of 24 h in a preliminary study. Those subjects with a particularly pronounced and those subjects with an only slightly pronounced reaction of the basal metabolic rate will be invited to the main study. Here, in random order (24 h fasting vs. 8 h fasting), the following is compared * how the basal metabolic rate of the body reacts to the reduced energy intake. * how the energy metabolism increases after a test meal * what role in particular the thyroid hormones play in this adaptation. In addition, a sample of the subcutaneous adipose tissue is taken in each case and it is examined how the regulation of metabolic processes at the cellular level.
Detailed description
The extent of this adaptation varies significantly between different individuals. The aim of this research project is to investigate how this adaptation of the metabolism is regulated by the body. For this purpose, we will first measure how the so-called basal metabolic rate of the body reacts to a short-term fasting of 24 h in a preliminary study. Those subjects with a particularly pronounced and those subjects with an only slightly pronounced reaction of the basal metabolic rate will be invited to the main study.
Interventions
OTHERmixed meal test
mixed meal test after fasting
Sponsors
University Hospital, Basel, Switzerland
ETH Zurich
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
randomized, open label, cross-over, monocentric trial in healthy volunteers
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Age: 18 to 40 years * Body mass index 18.0 to 27.0 kg/m² *
Exclusion criteria
* Chronic conditions necessitating medical treatment (e.g., renal failure, hepatic dysfunction, cardiovascular disease, diabetes mellitus), * Known or suspected non-compliance, drug or alcohol abuse, * Inability to follow the procedures of the study * Participation in another study with investigational drug within the 30 days preceding and during the present study, * Previous enrolment into the current study, * Enrolment of the investigator, his/her family members, employees and other dependent persons, * Hypothyroidism or hyperthyroidism * Pregnant, breastfeeding and menopausal women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| change of energy expenditure (EE) determined by indirect calorimetry (kcal per 24h) | 60 minutes after ingestion of test meal | Difference of EE (determined by indirect calorimetry) between baseline and 60 minutes after ingestion of the liquid test meal. Both measurements will be performed after an overnight fasting period. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Level of free thyroxine | after 24 hours and after 12 hours of fasting | The thyroid hormone (TH) system is an important regulator of EE. We hypothesize that levels of TH are higher in the spendthrift than in the thrifty group. |
| Level of free triiodothyronine | after 24 hours and after 12 hours of fasting | The thyroid hormone (TH) system is an important regulator of EE. We hypothesize that levels of TH are higher in the spendthrift than in the thrifty group. |
| Respiratory quotient (RQ) | after 24 hours and after 12 hours of fasting | We hypothesize that the participants in the thrifty group have a lower proportion of fatty acid metabolism and therefore a higher RQ. |
Countries
Switzerland
Contacts
Primary ContactMatthias Betz
Backup ContactRahel Loeliger
Outcome results
None listed