Endometrial Cancer, Endometrial Hyperplasia With Atypia, Endometrial Hyperplasia Without Atypia, Estrogen Receptor, Peripheral Blood Mononuclear Cells, Postmenopause, Progesterone Receptor
Conditions
Brief summary
A prospective observational study of endometrial tissue and peripheral blood mononuclear cells receptivity to sex steroid hormones in postmenopausal patients with endometrial proliferative processes
Detailed description
Endometrial cancer is in third place among cancer diseases in female population of Russia. The peak morbidity occurs during the postmenopausal period. In this regard, early diagnosis of previous endometrial proliferative processes and effective methods for their treatment are relevant. However, failures with hormonal therapy are often observed. This may be due to the low receptivity of the pathological tissue. It is also known that the functional activity of immunocompetent cells is controlled by the immune system, however, studies of the receptivity of peripheral blood mononuclear cells to sex steroid hormones were carried out in healthy blood donors. Changes in mononuclear cells receptivity may be one of the pathogenetic links in the development of endometrial proliferative processes and endometrial cancer. This may also influence the effectiveness of their treatment. In this regard, the purpose of the study is to evaluate the role of the expression of estradiol and progesterone receptor genes in endometrial tissue and peripheral blood mononuclear cells in the occurrence of endometrial proliferative processes in postmenopausal patients with a pathogenetic justification for the choice of treatment method. To achieve this goal, the investigators investigated the expression level of estrogen and progesterone receptors (ERa, ERb, GPER, PRA, PRB, mPR, PGRmC1) by RT-PCR in pathological endometrial tissue and peripheral blood mononuclear cells. GABDH was used as a comparison gene. The data obtained made it possible to determine the significance of mononuclear cell receptivity in the pathogenesis of endometrial proliferative processes.
Interventions
DIAGNOSTIC_TESTendometrial tissue and peripheral blood mononuclear cells receptivity
Participants investigated the expression level of estrogen and progesterone receptors (ERα, ERβ, GPER, PRA, PRB, mPR, PGRmC1) by RT-PCR in pathological endometrial tissue and peripheral blood mononuclear cells. GABDH was used as a comparison gene.
Sponsors
Pirogov Russian National Research Medical University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
53 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* endometrial polyps * endometrial hyperplasia * atypical endometrial hyperplasia * endometrial cancer
Exclusion criteria
* hormonal treatment (estrogen-progestogens, gestagens, gonadotropin-releasing hormone agonists, menopausal hormone therapy and tamoxifen) for 6 months before the study * uterine fibroids, larger than 6-7 pregnancy weeks * pathology of the uterine appendages according to ultrasound pelvis * inflammatory diseases of various localization at the time of taking the material
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| endometrial tissue and peripheral blood mononuclear cells receptivity | through study completion, an average of 1 year | Expression level of ERα, ERβ, GPER, PRA, PRB, mPR, PGRmC1 in endometrial tissue and peripheral blood mononuclear cells in patients with endometrial polyps, endometrial hyperplasia, atypical endometrial hyperplasia, endometrial cancer |
Outcome results
None listed