Inflammation, Post Operative Pain
Conditions
Keywords
Cryotherapy, Substance P, Apical periodontitis, Post Operative Pain
Brief summary
The aim of this clinical trial is to evaluate the effect of using intra-radicular cryotherapy technique in the form of irrigation with cold sodium hypochlorite The main questions to answer are: 1. Does Postoperative pain is different between the three groups? 2. Does level of substance P different between the three groups ?
Detailed description
* The patients will be randomly divided into three groups Group 1: control group (n=25) Regular root canal treatment with regular irrigant will be used. Group 2: (n=25) Intraradicular cryotherapy will be performed where : * Normal root canal treatment will be done with irrigation using sodium hypochlorite cooled to 2°C continuously during our mechanical instrumentation. Then a final flush will be done with cold saline 2°C for 5 minutes. Group 3: (n=25) Normal root canal treatment will be done with irrigation using sodium hypochlorite at room temperature during our mechanical instrumentation. Then a final flush will be done with cold saline 2°C for 5 minutes.
Interventions
OTHERcryotherapy
Cooling of irrigation
Sponsors
Ain Shams University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Patients that will be diagnosed with symptomatic apical periodontitis * Single-rooted premolars (radiographically and clinically assessed) * Medically free patients. * Teeth with mature apex
Exclusion criteria
* Patients with other pulpal diagnosis. * Medically compromised patients. * Pregnant females. * Patients taking analgesics or anti-inflammatory drugs.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Level of post operative pain | after 6 hours, 24 hours, 48 hours, 72 hours | On visual analogue scale (VAS) questionnaire, patients will report their level of pain 0° refers for no pain, and the 100° degree refers for unbearable pain |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Level of substance P | After access cavity, after finishing mechanical perpetration, before obturation(after 3 days) | apical fluid sample will be taken using sterilized, medium sized paper point (Diadent Korea, Seoul, Korea) will be inserted into the periapical area and left in place for 30 seconds and the sample will be placed into a 1.5-mL Eppendorf tube containing 100 \_L of Tris-HCl buffer, pH 7.5, with 0.15 M NaCl and 1 mM CaCl2; the tubes will be placed on a shaker at room temperature for 3 hours and then stored at -70°C |
Countries
Egypt
Outcome results
None listed