Female Infertility
Conditions
Brief summary
The present research project aims to study the DNAm mechanisms underlying the reduction of fertility due to the progressive depletion of oocyte quality. Specifically, our project aims to build an epigenetic clock for MGCs by using outcomes that are certainly related to female fertility. The validation of such findings will be carried out on peripheral blood in order to guarantee its non-invasiveness and allow for any clinical transferability. In order to identify a blood epigenetic signature able to predict female infertility, we planned to explore the problem from different points of view by conducting several studies in different settings.
Interventions
GENETICsearch for epigenetic profile of infertility
examine the epigenetic profile of DNA extracted from MGCs and blood sample
Sponsors
Università Vita-Salute San Raffaele
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 43 Years
Healthy volunteers
Yes
Inclusion criteria
* 18\< age \<43, Infertile woman undergoing ART, recurrent pregnancy loss
Exclusion criteria
* age \< 18 or \> 44, previous ovarian surgery, severe male factor infertility
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| differences of DNA methylation in granulosa cells of patients undergoing ART | 3 years | The epigenetic profile of DNA extracted from granulosa cells of patients undergoing ART (COHORT1) will be examined to identify position and region's differences of DNA methylation in pregnant women (fertile women) and in who does not get pregnant (infertile women) |
| differences of DNA methylationin the peripheral venous blood of patients undergoing ART | 3 years | search for this methylation difference found in the granulosa cell sample, also in the peripheral venous blood of these same patients |
| differences of DNA methylationin the peripheral venous blood of women attempting to conceive naturally | 3 years | The specific epigenetic modification will be searched in the peripheral venous blood of women attempting to conceive naturally (COHORT2) |
| differences of DNA methylationin the peripheral venous blood of new patients undergoing ART | 3 years | The specific epigenetic modification will be searched in the peripheral venous blood of two new cohorts of patients referred to PMA centres. |
| impact of exogenous pro-inflammatory factors | 3 years | on all the above cohorts will be investigated, via online questionnaire |
Countries
Italy
Contacts
Primary ContactValeria Stella Vanni
Outcome results
None listed