Aging, Healthy
Conditions
Brief summary
The goal of this basic science clinical trial is to understand whether adipose tissue blood flow changes between younger and older healthy adults. The main question\[s\] it aims to answer are: * Is basal and nutrient responsive adipose tissue blood flow (ATBF) different between younger and older adults * What molecular and systemic signatures related to adipose tissue blood flow are altered between these two groups. Participants will undergo measurements of adipose tissue blood flow using the xenon washout technique, undergo 2 abdominal subcutaneous adipose tissue biopsies, and drink a sugary drink.
Detailed description
On Visit 1, volunteers will provide written informed consent (and urine pregnancy test for women of childbearing age), complete a Dual x-ray absorptiometry scan, baseline labs (comprehensive metabolic panel) and a cardiopulmonary exercise test. Participants will arrive fasted at 08:00, and urine pregnancy test for women of childbearing age administered and an intravenous catheter will be placed. Following 30min of supine rest, baseline venous blood samples will be collected. ATBF will be assessed on participants' left abdomen first by measurement of blood flow using 133Xenon washout method. Immediately thereafter, an abdominal adipose tissue biopsy of the left side will be performed but ≥6 cm distant from location of 133Xenon injections. Subjects will them consume 75 gm glucose; because ATBF peaks 30-60 minutes following ingestion of glucose, ATBF measures will start 30 minutes after ingestion on the contralateral side followed immediately by plasma measures and another adipose tissue biopsy. During ATBF measurements, brachial blood pressure will be assessed in two-minute intervals.
Interventions
OTHER75 gm oral glucose load
Consumption of 75 gm oral glucose load in order to test nutrient responsiveness
Sponsors
Mayo Clinic
National Center for Advancing Translational Sciences (NCATS)
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Within the ages of 18-35 years or 65-80 years at time of study visit * BMI between 20-25.0 kg/m2.
Exclusion criteria
* Presence of diagnosed cardiometabolic diseases (e.g., Type 2 diabetes, hypertension, Heart Failure) * Taking prescription anticoagulants * Taking prescriptions or supplements that effect adipose tissue metabolism (i.e., statins, Thiazolidinediones, niacin, atypical antipsychotics, or fish oil) * Pregnant or nursing * Inability to provide written informed consent * Smokers
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Adipose tissue blood flow | Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load | blood flow will be measured in the upper body subcutaneous adipose tissue using 133 Xenon washout and reported as blood flow in ml/min/100g adipose tissue. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Genomic alterations | Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load | RNA-Seq from adipose tissue biopsies |
Countries
United States
Contacts
PRINCIPAL_INVESTIGATORKelli Lytle, PhD
Mayo Clinic
Outcome results
None listed