Oral Cancer, Oral Premalignant Lesions as Leukoplakia and Lichen Planus
Conditions
Keywords
oral cancer, saliva, serum, dipeptidyl peptidase, potentially malignant lesions.
Brief summary
Aim: The current study targets linking serum and salivary dipeptidyl peptidase-4 with oral squamous cell carcinoma and comparing it with potentially malignant lesions and control to validate dipeptidyl peptidase-4 as a diagnostic marker for early detection of oral cancer and to reveal its possible role in carcinogenesis. Methodology: A total of 45 patients were recruited and subdivided into 2 groups: Group I: 15 patients having oral squamous cell carcinoma. Group II: 15 patients with potentially malignant lesions (leukoplakia and oral lichen planus) compared to 15 systemically healthy participants having no oral mucosal lesions acting as a control group (Group III). Serum and whole unstimulated salivary samples were collected from all participants to evaluate dipeptidyl peptidase level in different groups using enzyme linked immune-sorbent assay (ELISA) kit. ROC analysis was done to reveal area under the curve, sensitivity, specificity and diagnostic accuracy of DPP-4 among different groups.
Interventions
DIAGNOSTIC_TESTserum and unstimulated salivary samples
Participants were told to cease smoking, eating, or drinking before the collection of samples by about half an hour. Obtaining the sample was done by demanding the individual to swallow then incline his head forward to expectorate saliva in a sterile tube for about 3 min. Afterwards the samples were coded by serial numbers then stored at -20°C until being assessed. Under aseptic circumstances and using plain tubes, 5ml peripheral venous blood samples were collected from all participants by standard venipuncture. Samples were transported to biochemistry laboratory and centrifuged followed by filtration of the clarifying supernatant and then stored at -20ºC
Sponsors
Fayoum University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
30 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* systemically healthy * No current medication, * both genders with age ranging from 30 to 65 years old. * having oral leukoplakia, oral lichen planus or oral squamous cell carcinoma
Exclusion criteria
* systemic disease * pregnancy * lactation * being diagnosed with any other oral mucosal lesion
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| DPP-4 level detection | level of DPP-4 is measured after the completion of all sample collection carried at the day of enrollment of subjects in the investigation after diagnosis confirmation | level of DPP-4 is measured in all collected serum and salivary samples |
Countries
Egypt
Outcome results
None listed