The Systemic Concentration of Short-Chain Fatty Acids After Delivery in the Small and Large Intestine of Healthy Volunteers

The Systemic Availability of Short-Chain Fatty Acids After Delivery in the Small Intestine and the Colon

Status

Completed

Phases

Unknown

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT06082726

Acronym

Sistine

Enrollment

Registered

2023-10-13

Start date

2023-10-09

Completion date

2024-03-11

Last updated

2026-02-23

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Systemic Availability, Intestinal Absorption, Metabolism, Short-chain Fatty Acids

Brief summary

The goal of this crossover study is to evaluate the systemic availability of short-chain fatty acids (SCFA) that are either administered in the small intestine or the colon in healthy volunteers. The main question it aims to answer is whether the site of administration affects the amount of SCFA that reaches the systemic circulation. On two test days participants will ingest capsules filled with SCFA that are specifically delivered in the small intestine or the colon. After ingestion, blood samples will be collected at regular time points.

Interventions

DIETARY_SUPPLEMENTSCFA

Participants will ingest the capsules during a standard no fiber breakfast. A primed continuous infusion containing SCFA will run during the whole test day. Blood sampling will continue for 12 hours.

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

BASIC_SCIENCE

Masking

SINGLE (Subject)

Eligibility

Sex/Gender

ALL

Age

18 Years to 50 Years

Healthy volunteers

Yes

Inclusion criteria

* female and male * healthy participants * age within 18 - 50 years * normal BMI (18.5-25 kg/m\^2)

Exclusion criteria

* Chronic gastrointestinal disorders such as inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), celiac disease, chronic constipation (less than 3 stools a week) and chronic frequent diarrhoea (more than 3 stools a day) * Previous abdominal surgery, except from appendectomy * Being on a weight loss, gluten-free, lactose-free, or vegan diet * The donation of blood during the last 3 months or suffering from low blood haemoglobin levels * The use of antibiotics or other medication that affects the gastrointestinal tract 3 months preceding the study and/or during the study * The use of prebiotics or probiotics 2 weeks preceding the study and/or during the study * Pregnancy, lactation or wish to become pregnant during the study period * Previous or current substance/alcohol dependence or abuse (\> 2 units per day/14 units per week)

Design outcomes

Primary

Measure	Time frame	Description
Concentration of 13C-short-chain fatty acids in blood	up to 12 hours	Assessed by analysing SCFA in blood samples collected at regular time points

Countries

Belgium

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 24, 2026