Diabetes Mellitus, Type 1
Conditions
Brief summary
The aim of the study is to analyse the biomechanical stiffness of red blood cells extracted from subjects with type I diabetes with and without microvascular complications through a biophysical characterization of red blood cells using microscopy, spectroscopy and scattering measurements. 48 subjects with type 1 diabetes, 24 with and 24 without microvascular complications will be enrolled. From all subjects a single blood sample will be taken to perform the analysis of red blood cells morpho-mechanical properties.
Interventions
DIAGNOSTIC_TESTBlood test - atomic force microscopy in the force spectroscopy mode
A single blood test to be analysed through atomic force microscopy in the force spectroscopy mode
Sponsors
Fondazione Policlinico Universitario Agostino Gemelli IRCCS
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
50 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* Diagnosis of type 1 diabetes for at least 10 years * Stable metabolic control (HbA1c \<7.5%) for at least 6 months * BMI between 18.5 and 30 Kg/m\^2 * Systolic blood pressure \< 140 mmHg and diastolic blood pressure \<90 mmHg
Exclusion criteria
* Blood pressure higher than 140/90 mmHg * Unstable metabolic control (HbA1c \>7.5%) * BMI \> 30 Kg/m\^2 or \< 18.5 Kg/m\^2 * Liver failure * Concomitant acute infections * Uncontrolled thyroid diseases * Pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| In 48 subjects with type 1 diabetes with and without microvascular complications, the average stiffness of red blood cells, measured using atomic force microscope and quantified using Young's modulus, will be measured. | 1 day | Young's modulus values are obtained using atomic force microscopy |
Contacts
Primary ContactDario Pitocco
Outcome results
None listed